文库

基本解释 (translations)

omnibuses

词组短语

a series of books issued in a single format by a publisher

与文库相关的网络例句 [注：此内容来源于网络，仅供参考]

Improvement of Residue by Hydrocracking with Solid Acid, Solid Base and Radical Initiators

固体酸／碱和引发剂促进渣油轻质化的初步研究，石斌；任振东；门秀杰；方日强；阙国和-66文免费论文库，大学生论文库

To our knowledge,this is the the first BAC library for this newly introduced model organism.

就我们所知，本文库是这种新型模式生物在国际上的第一个BAC文库。

Results (1) The forward and reverse subtracted cDNA libraries of different metastastic potential large cell lung cancer cell lines were successfully constructed;(2) With blue-white colony and dot blot, 307 positive clones in the forward subtracted library and 78 positive clones in the reverse subtracted library were obtained;(3) 55 clones were successfully sequenced in the forward subtracted library. Homolog analysis confirmed 23 differential expression segments, which were similar to the human genes already known, including NME2, NPM1, MT 2A, HSPE1, TAFIA, EPRS, PX19 and EIF3S9 et al;(4) 31 clones were successfully sequenced in the reverse subtracted library. Homolog analysis confirmed 16 differentially expressed segments. 15 of them were similar to the human genes already known, including ANXA2, TUBB, PKN2, GNAS, EEF1A1, SSR2 and RPLPO et al. Only one segment had partial homology to known human genes. This segment was supposed to be the new EST segments which might not have been cloned.

结果(1)成功构建人大细胞肺癌高低转移株差异表达基因正向消减cDNA文库和反向消减cDNA文库；(2)经蓝白菌落筛选和斑点杂交，正向消减文库获得307个阳性克隆，反向消减文库获得78个阳性克隆；(3)正向消减文库挑选55个克隆成功测序，经同源性分析最终确定差异表达基因片段23个与已知人类全长基因具有很高相似性(95％～100％)，这些基因包括NME2、NPM1、MT2A、HSPE1、TAFlA、EPRS、PX19和EIF3S9等；(4)反向消减文库挑选31个克隆成功测序，经同源性检索和比对最终确定差异表达基因片段16个，其中15个与人类全长基因具有很高相似性(95％～100％)，包括ANXA2、TUBB、PKN2、GNAS、EEF1A1、SSR2和RPLPO等基因。1个片段和己知人类基因仅有部分相似性，表明它可能是未被克隆的人类基因EST片段。

Using semi-quantitative RT-PCR, the differential expression profiles of eleven selected genes were confirmed in the ovaries of triploid and diploid. These genes fell in gene categories with a wide range of functions. The results indicated that triploidy affects the dynamic gene regulatory network in triploid ovary. This study established a firm basis for future investigation on characterization of crucial molecular events for normal ovarian development in shrimp.To further dissect exact gene functions for gonad development of shrimp, three differentially expressed genes between diploid and triploid ovary, PCNA (proliferating cell nuclear antigen), CAS/CSE1 (cellular apoptosis susceptibility protein/chromosome segregation 1) and SSRF (spermatogonial stem-cell renewal factor) were characterized on certain aspects.

利用抑制性消减杂交技术，建立了对虾二倍体和三倍体卵巢间的2个消减文库；在正向消减文库(以三倍体卵巢作为实验组，二倍体卵巢作为驱动组)中，鉴定到54个基因；在反向消减文库(以二倍体卵巢为实验组，三倍体卵巢为驱动组)中，鉴定到16个基因；选取11个差异表达的基因，利用半定量RT-PCR的方法对其在二倍体和三倍体卵巢间的表达进行了检测，均能很好地与消减结果相吻合；这些差异基因编码多种功能的蛋白，分析表明染色体的三倍化使三倍体卵巢中的基因调控网络受到了影响；为深入揭示维持卵巢正常发育的关键分子调控事件奠定了基础。

Abstract］ Objective: To explore the method for repairing tympanic membrane perforation due to chronic suppurative tympanitis. Method: 50% trichloroacetic acid solution was used to ignite the margin of the old tympanic perforation, then MEBO patch was applied on the tympanic membrane.

核心提示：《赛蝶论文库》近期推出免费论文库，所有论文均来自网络或作者自行发布，版权归原作者所有，这些免费论文能够起到启迪思维的目的，欢迎本站的论文库和范文库的文章。

The principle of verbalism that becomes worldwide practices principles of contemporary legal society is main based on that it can guarantee participants contesting on court trial, mean while, it also embody the procedure reasonableness as well as guarantee the case's facts to be disclosed.

核心提示：《赛蝶论文库》推出免费论文库，包含教学论文和毕业论文，所有论文均来自网络或作者自行发布，版权归原作者所有，这些免费论文能够起到启迪思维的目的，欢迎本站的论文库和范文库的文章。

The vocalism is a comprehensive art, which the process of study singing is a pocess of enhancement and development.

核心提示：《赛蝶论文库》推出免费论文库，包含教学论文和毕业论文，所有论文均来自网络或作者自行发布，版权归原作者所有，这些免费论文能够起到启迪思维的目的，欢迎本站的论文库和范文库的文章。

The pHybLex/Zeo-Idl plasmid and the cDNA library plasmid were sequentially transformed into the yeast swains and screened to obtain Leu2^＋ and Leu2^＋LacZ^＋ clones, with the false positive clones excluded using positive and negative controls, and the plasmid of the true positive clone was sequenced and blasted for homological analysis.

方法PCR方法扩增Id1的编码序列并定向克隆入诱饵质粒pHybLex/Zeo，构建重组诱饵质粒pHybLex/Zeo-Id1，酶切鉴定后转化入酵母株EGY48/pSH18-34，检测重组诱饵质粒有无非特异激活报告基因Leu2、LacZ作用；扩增并提取成人肺组织文库质粒，将文库质粒及重组诱饵质粒转化入酵母细胞，依次筛选Leu2^＋，Leu2^＋LacZ^＋克隆；设置阴性及阳性对照，重复筛选Leu2＋LacZ＋克隆并排除假阳性克隆，获取真阳性文库质粒，进行测序和同源性比对。

The capacity of the primary library was 5.0×105 PFU/mL and the capacity of the amplified library was 8×109 PFU/mL. The cDNA library was immunoscreened by the rabbit serum against H.qinghaiensis larval tick, the positive pluques were subcloned, and their plasmids extracted and then transformed into Escherichia coli JM109. Eventually, 11 genes were obtained and designated HqL1,HqL2,HqL3,HqL4,HqL5,HqL6,HqL7,HqL8,HqL9,HqL10 and HqL11,respectively.

采用常规方法构建了青海血蜱幼蜱cDNA表达文库，初级文库的库容量为5.0×105PFU/mL，扩增后的库容量为8×109PFU/mL；用兔抗青海血蜱幼蜱阳性血清对该文库进行了免疫学筛选，对阳性噬菌体进行了亚克隆，提取质粒后转化宿主菌JM109，最终共得到幼蜱基因11个，分别命名为HqL1、HqL2、HqL3、HqL4、HqL5、HqL6、HqL7、HqL8、HqL9、HqL10、HqL11。

Ridgeia piscesae,living around the hydrothermal vent of Juan de Fuca Ridge,isan ideal model for studying the adaptative mechanism to extreme environment.Forinsights of its molecular characteristics,a SMART cDNA library of 5.71×10~6 cloneswas constructed.

本研究以深海热液区Juan de Fuca Ridge的典型生物管状蠕虫Ridgeiapiscesae为研究对象，运用SMART技术构建了其cDNA文库，各项指标均符合文库质量标准：文库的容量为5.71×10~6个克隆，平均插入片段大小～1.0kbp。

与文库相关的网络解释 [注：此内容来源于网络，仅供参考]

antibody library：抗体文库

antibody fusion protein抗体融合蛋白(质) | antibody library抗体文库 | antibody production抗体产生

Athenaeum：文库

戈尔盖 Gorguet, Auguste | 文库 Athenaeum | 文学中的维多利亚时代 The Victorian Age in Literature

DS Dengeki Bunko: Alison：(日本) (DS电击文库:艾莉森)电子小说类

0743 - Power Pro Kun Pocket 9 (日本) (... | 0744 - DS Dengeki Bunko: Alison (日本) (DS电击文库:艾莉森)电子小说类 | 0745 - Simple DS Series Vol. 11: Mou Ichido Kayoeru: The Otona no Shougakkou (日本) (S...

CAAC CAKD：会议论文库

1 book 图书 | 2 CAAC CAKD会议论文库 | 3 CAAD CAKD博硕论文库

reference library：参考文库

参考图书馆/reference library | 参考文库/reference library | 参考性意见/advisory opinion

subtracted library：扣除文库

差示文库(differential library)或扣除文库(subtracted library), 是反映不同组织细胞或同一种细胞在不同功能状态下基因表达差异的cDNA文库. 用于研究细胞的发育、分化、细胞的分裂周期、细胞对药物和生长因子的诱导反应以及肿瘤等疾病的分子基础.

subtracted libmrary：扣除文库

subtilisin 枯草(杆菌)蛋白酶 | subtracted libmrary 扣除文库 | subtracted probe 扣除探针

doctorial dissertation center：博士论文文库 n.(学位)论文

reader's card office 办证处 | doctorial dissertation center 博士论文文库 n.(学位)论文 | master essay center 硕士论文文库

genomic library：基因组文库

7、基因组文库:(genomic library)整个生物体基 因组随机产生的重叠DNA片段的克隆的总和8、基因库:gene bank 某一生物群体中全部个体 所共有的全部基因 9、蛋白质组:proteome 在某种细胞或组织中整个 基因组所产生的各种蛋白质的组合.由基 因组编码的全部蛋白质总合 10、蛋白质组学:proteomics 研究一个基因组中基 因编码的

genomic library：基因文库

的筛选和鉴定 目的基因(target DNA)的制备 目的基因 目的基因(外源基因) 目的基因(外源基因) 制备基因组DNA 制备基因组 - 基因文库(genomic library)-存在于转化细菌 基因文库 存在于转化细菌 克隆载体携带的所有基因组DNA的集合中,