used ['ju:st]

used的基本解释

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[变形] use的动词过去式

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[变形] use的过去分词

adj.

习惯的, 使用过的, 二手的

• My brother bought a used car.
• 我弟弟买了一部旧车。

• The astronauts soon got used to the condition of weightlessness.
• 太空人很快就习惯了失重状态。

• I am used to walking to school.
• 我习惯走路上学。

used的同义词

v. apply · use

used的反义词

adj. unused

与used相关的情景对话

衣食住行 / 搬家

A:

I could never (get used to/ grow accustomed to) it, though.

可是我（无法习惯/不能习惯）。

B:

It’s not too bad

还不是很糟糕。

时事热点 / overdose-(吸食过量)

A:

Did you hear about John?

听说了汤姆的事情吗？

B:

No. What happened?

没有，什么事？

A:

He overdosed on heroin.

他服用海洛因过量。

B:

Oh my god. He used drugs?

哦，天。他吸毒？

A:

I guess so. Supposedly, it was the first time he did heroin.

我想是。据称，这是他第一次吸食海洛因。

B:

His poor family.

他的家人真可怜。

A:

Yeah.

是呀。

衣食住行 / 介绍

A:

Mary, this is Joe's brother David.

玛丽，这是乔的兄弟大卫。

B:

I'm very glad to meet you.

我很高兴见到你。

A:

It's a pleasure to meet you.

很高兴见到你。

B:

How do you like Beijing so far?

到现在你觉得北京怎么样？

A:

It's really different from what I expected.

与我想象的很不一样。

B:

Don't worry. You'll get used to it in no time.

别担心。你很快就会习惯它了。

与used相关的网络例句 [注：此内容来源于网络，仅供参考]

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

Achieve a special aesthetic culture of Japan in the world, Japanese men have used aesthetic culture to control and judge class or age and social position of Japanese women, KOGAI MAGE and TAKASHIMADA MAGE is used by common women, KATSUYAMA MAGE and TENJIN MAGE is used by geshia women, HYOKO MAGE is used by concubine, RAKAKU MAGE is used by servant, SANRIN MAGE is used by young women, KUMEZO MAGE is used by a middle-aged women, MARU MAGE is used by women of old age.

日本古代发型是最足以代表日本风情的民族发式，翻开日本古代发型的演变史；最早可推到3世纪的古坟时代，历经千年以上之改良创新，而发展出多彩多姿的款式。皇族之笄髷及上流社会之高岛田髷，女医及巫女等特殊职业之片手髷，普通国民流行之桃割髷及银杏髷，艺妓专用之胜山髷及天神髷，女妾专属之兵库髷，女佣之螺角髷；少妇的三轮髷；中年之久米三髷；老年之丸髷，均有各领风骚的发型区别，毛发款式描法依照人体脸型七种样式来美化。

When motion is towards the location of the addressee,come is much preferable to go in English while both laiand quare common in use in Chinese.When motion is towards the location of the addressee at a past time,laimust be used in Chinese if the addressee is there at the time of utterance while qumust be used if the addressee is not there at the time of utterance.By contrast,come is used in English either when the addressee is there or when he is not there at the speaking time,while go is only used when the addressee is not there at the time of utterance. When both the speaker and the addressee serve as the figure,which is indicated by the phrases like with me/us/you in English and the phrases like he ni,he nimen,gen wo,gen womenin Chinese,come is much preferred to go for the figure"s motion,while in the same context,quis used for the figure"s motion.What"s more,come is more frequently used to indicate the figure"s movement towards a place where neither the speaker nor the addressee is located at the time of utterance,while quis often used in Chinese in the same case.

当移动体朝向听话人所在方位移动时，英语多用come，汉语可用"来/去"；当移动体在一个过去的时间朝向听话人所在方位移动时，汉语可用"来"表示听话人在说话时间仍在位移参照点，用"去"表示听话人在说话时间已不在位移参照点，而英语在两种情况下都可用come，用go则表示听话人在说话时间已不在位移参照点；当说话人和听话人一起作为移动体而句中主语只是说话人或听话人一方时，英语多用come，汉语多用"去"。

与used相关的网络解释 [注：此内容来源于网络，仅供参考]

used：用过了的

数据不应该是生产出来的(produced),精选出来的(picked),用过了的(used)数据便没有了意义. pick精选, 摘下;produce生产,制造;use使用;gather收集,搜集,整理. 在不远的将来,水供应的预报和水的使用将(will)很可能依赖的是人们对高山积雪的认识,

used：已用

6、至此,分区全部创建完毕,但"已用"(Used)都还是"未知"(unknow),如果不满意,还可以点击"撤销对分区设置的修改"或"后退"进行更改. 如果确定,就点"前进"继续安装进程. 文档查看器(Evince)是一个PDF文件阅读器,但默认设置对中文的支持不够好,

used：已使用

在里程券正面盖上"已使用"(USED)章.并将其附在会计联后面,交结算5) 在里程奖励券正面加盖"已使用"(USED)章.2, 在里程奖励券正面加盖"已使用"(USED)章.3, 在里程奖励券正面加盖"已使用"(USED)章,填写里程银行升舱兑换3,

used：使用

但应该警告粗心的读者,在管理语言这种语言中,当东西可以被"利用"(leveraged)时,就很少用"使用"(used)来表达;"变化"这个词之前总是有"海量"(sea)、"梯级"(step)或"量子"(quantum)等词;而且科技总是"尖端的"、"出众的"或"先进的"

used to：过去常常

167)be used to 习惯于 | 168)used to 过去常常 | 169)wake...up 唤醒