maturation stage
- maturation stage的基本解释
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[医] 成熟期
- 相似词
- 更多 网络例句 与maturation stage相关的网络例句 [注:此内容来源于网络,仅供参考]
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The results show that: 1 supplementation of protein to maturation media improves cumulus expansion in vitro compared to the protein-free control, but cumulus expansion is not necessarily related to oocyte nuclear maturation in pigs, and cumulus expansion is not the criterae for determination of nuclear maturation of pig oocytes, but only the exclusion of the first polar body; 2 exposure of pig COCs to hormone supplements for 23-24 hours improved cumulus expansion but had no significant effect on nuclear maturation compared to that for 46-48 hours; 3 under our research conditions, supplementation of different proteins into different maturation media has different effects on porcine oocyte nuclear maturation, but has no significant effect on subsequent embryonic development after IVF; 4 the nuclear maturation rates of pig oocytes matured in mTCM+pFF and mNCSU+pFF are superior than that in mNCSU+FCS; 5 different maturation media have no effect on pig oocyte cumulus expansion and subsequent embryonic development after IVF.
结果显示:(1)在成熟液中添加蛋白质可以加强卵丘细胞的扩散,但猪卵母细胞的核成熟与其周围的卵丘细胞扩展没有必然的联系,卵丘细胞扩散或成放射状不宜作为猪卵母细胞核成熟的标准,只有排出第一极体才能作为猪卵母细胞核成熟的标志;(2)在猪COCs的46-48小时成熟培养的后23-24小时阶段去除成熟液中的激素不但可以保证卵母细胞的核成熟率,而且可加强卵丘细胞的扩散;(3)在现有实验条件下,在mTCM和mNCSU中添加10%pFF与在mNCSU中添加10%FCS相比可获得较高的猪卵母细胞核成熟率;(4)在不同的成熟液中添加不同的蛋白质对猪卵母细胞核成熟率的影响效果不一样,但对体外受精后的早期胚胎发育影响不明显;(5)成熟液种类对猪卵母细胞的卵丘细胞扩散和体外受精后的早期胚胎发育无显著影响。
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The results showed that the rates of mature oocyte were 75.2%, 73.1%, 69.8%, 63.5%and oocyte at telophase of MI were 16.3%, 15.9%, 16.9%, 27.0%, respectively. The rates of maturation of oocytes cultured in TCM199 with serum, EGF and TGFαwere significantly higher than that of with BSA team (P<0.05) and the number of oocyte stayed at telophase of MI in TCM199 with serum, EGF and TGFαteam were significantly less than that with BSA team(P<0.05); Significant higher rates (66.6%, 66.6%, 73.6%) of normalα-tubulin distribution in oocytes cultured in TCM199+ serum, EGF and TGFαwere compared to that of TCM199+BSA(43.3%)(P<0.05). The rates of oocyte with cortical granules in cortex were 58.8%, 33.9%, 54.7%and 47.9%respectively, there was significant difference between oocytes cultured with serum, EGF and BSA(P<0.05). In conclusion, TGFαand EGF can promote the oocyte nuclear transition from telophaseⅠto metaphase of meiosisⅡ, and improved the expression and distribution ofα-tubulin during the ovine oocytes maturation; EGF and serum could promote oocyte cytoplasm maturation. The results suggested that EGF and TGFαmight substitute some substance in serum to improve the quality of oocyte nucleus maturation in vitro, but EGF might be more functional than TGFαto promote the maturation of ovine oocyte ooplasm.
三、EGF和TGFα对卵母细胞成熟的影响未成熟卵母细胞分别在TCM199基础培养液+血清、TCM199基础培养液+BSA+EGF、TCM199基础培养液+BSA+TGFα和TCM199基础培养液+BSA四组成熟培养系统中成熟培养,22小时后上述各组卵母细胞的核成熟率分别为75.2%、73.1%、69.8%、63.5%,处于第一次减数分裂末期的比率分别为16.3%、15.9%、16.9%、27.0%,在添加血清、EGF、TGFα各组中核成熟率显著高于其它各组(P<0.05),处于末期的比例明显低于其它各组(P<0.05):添加血清、EGF、TGFα各组在a-Tubulin蛋白的分布与表达上(66.6%、66.6%、73.6%)明显高于BSA组的正常率43.3%(P<0.05);各组CG发生迁移较好的卵母细胞的比率分别为58.8%、33.9%、54.7%、47.9%,血清组和添加EGF组CG迁移至皮质区的比例明显高于仅添加BSA处理组(P<0.05)。
- 更多网络解释 与maturation stage相关的网络解释 [注:此内容来源于网络,仅供参考]
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maturation period:成熟阶段
maturation learning principle 成熟学习原则 | maturation period 成熟阶段 | maturation phase 成熟期
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maturation division:减数分裂,成熟分裂
成熟(期),生脓 maturation | 减数分裂,成熟分裂 maturation division | 成熟期 maturation phase
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maturation division:细胞成熟分裂,成熟分裂
mattress suture 褥式缝合法 | maturation division 细胞成熟分裂,成熟分裂 | maturation factor 成熟因子