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Methods The resistance of Salmonella isolates were detected by drug susceptibility tests with Kirby Bauer diffusion method. PCR was designed to detect tetA, tetB, tetC, tetD, tetE, tetG and tetK. The amplified products were sequenced and alignment analysis was performed.

用KB法测定分离株对四环素等21种抗生素的耐药情况，用PCR方法检测四环素耐药基因tetA、tetB、tetC、tetD、tetE、tetG及tetK，并对扩增产物进行测序。

Methods resistance of Salmonella isolates were detected by drug susceptibility tests with KirbyBauer diffusion method. PCR was designed to detect tetA, tetB, tetC, tetD, tetE, tetG and tetK. The amplified products were sequenced and alignment analysis was performed.

用KB法测定分离株对四环素等21种抗生素的耐药情况，用PCR方法检测四环素耐药基因tetA、tetB、tetC、tetD、tetE、tetG及tetK，并对扩增产物进行测序。

And then,the soluble target protein was purified with Ni-NTA column,and its C-terminal was sequenced with mass spectrum analysis.13 amino acids of C-terminal is identical to the Plasmodium falciparum TCTP when blasted in GenBank. Plasmodium falciparum ; Translationally controlled tumor protein ; Cloning expressing

虽然报道认为TCTP具有钙结合、金属内环境稳定、细胞内信号传导、作为IgE依赖性组胺释放因子、作为青蒿素类药物受体蛋白(Bhisutthibhanet al。，1998)和在约氏恶性疟原虫青蒿类药物抗性系中过表达等功能，但其具体生物学功能尚待进一步研究阐明

Although only 246bp sequenced in domain I, transition,transversion and percentage of divergence are higher than domain III.

灰雁和鸿雁mtDNA控制区序列转换数远大于颠换数，表明家鹅线粒体控制区序列在种间、控制区区域间表现出不同的变异模式。

The gene segment in 18S ribosome RNA gene of four Trichinella isolates were cloned and sequenced.

方法通过RT-PCR方法，克隆4个不同旋毛虫隔离种的18SrRNA基因片段，并进行比较分析。

The mtDNA COI, mtDNA COⅡand mtDNA Cyt b genes of different Trichogramma species and different geographical populations of the same Trichogramma species were sequenced, and the genetic distance and phylogenetic relationships of Trichogramma were respectively established based mtDNA gene sequences.

本研究成功测得了多种赤眼蜂不同种和不同地理种群mtDNA（COI、 COⅡ和Cyt b ）的基因序列，对赤眼蜂mtDNA基因序列从遗传距离和系统进化树进行分析，结果表明3个基因序列均适合赤眼蜂种一级的分子鉴定，不能用做种下阶元的区分，同时确定mtDNA Cyt b 基因为赤眼蜂蜂种鉴定的理想基因序列。

To study the taxonomy position of two Trypanosoma evansi strains from buffalos in Guangxi and Hubei provinces, the ITS (ITS1-5.8S-ITS2) gene of the T. evansi were amplified by PCR and sequenced.

为研究从水牛分离到的伊氏锥虫广西株和湖北株的分类学地位，对2株伊氏锥虫的核糖体基因内转录间隔区(ITS1-5.8S-ITS2)基因进行了克隆和测序分析。

To investigate the mechanism of wood formation and lignin biosynthesis, it is important to understand the function and characteristic of OMT. In the present study, a set of primer was designed based on the conserved region of OMT genes from several woody and non-woody plants, to clone the counter part gene by PCR and RACE from Chamaecvparis formosensis Matsum., Chamaeeyparis obtusa var. Jormosana, Abies kawakarnii, Tsuga ehinensis var. Jormosana and Taiwania eryptonierioide Hayata. The cloned genes were sequenced and their amino acid sequences predicted.

我们自资料库中收集并分析已发表之草本和木本植物caffeoyl CoA3-O-methyltransferase序列，在具高保守性的序列中设计了一对引子，并对红桧、台湾扁柏、台湾冷杉、台湾铁杉和台湾杉进行OMT基因的钓取，同时配合RACE的策略，成功获得红桧、台湾扁柏、台湾杉的全长基因及台湾冷杉与台湾铁杉之部分基因片段。

Mitochondrial 16S rRNA gene fragments were amplified from dolphins. Tursiops truncatus , Sousa chinensis and Steno bredanensis using PCR technique. The PCR products were about 600 bp and were directly sequenced subsequently.

摘 要：采用聚合酶链式反应技术扩增瓶鼻海豚、中华白海豚和糙齿海豚的线粒体DNA 16S rRNA基因，获得了大约600 bp的片段。

The cDNA inserts of these clones were sequenced and typical and untypical NLS were identified.

从文库筛选出来的基因中，选取了两个基因，分别代表已知和未知、有典型NLS和无典型NLS。

Chinese traditional virtue is humility and wariness,the compliment and praise to the others should be declination

中国人的传统美德是谦虚谨慎，对别人的恭维和夸奖应是推辞。如

We bought this house on the never-never.

我们以分期付款的方式买下这座房子。