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Personality traits were evaluated by Eysency Personality Questionnaire that was compiled by Eysency and revised by Gong. Standard scores of Psychoticism, Extraversion and Neuroticism were calculated, and taking 50 points as a boundary. Each dimension was assigned into 2 grades non-psychotic type (P0) psychotic type (P≥50), introversion type (E0), extraversion type (E0), non-nervous type (N0) and nervous type (N≥50). 5 mL ulnar vein blood was obtained without adding decoagulant, and then serum was removed. Genome DNA was extracted from blood clot by conventional chloroform for PCR analysis. PCR product was sequenced by pyrophosphoric acid. SNP was determined by PSQ96 MA sequenator. Allele frequency was analyzed by mass spectrometry.

人格评定采用Eysency编制，龚耀先修订的艾森克人格问卷、计算精神质，内外问，神经质3个个性维度的标准分，并以50分为界，将各维度分为两个等级：非精神病倾向型（精神质0）、精神病倾向型（精神质≥50）；内倾型（内外向0），外倾型（内外向≥多50）；非常经质倾向型（神经质0），神经质倾向型（神经质≥50）取肘静脉血5 mL，不加抗凝剂，弃血清，血凝块常规酚氯仿提取基因组DNA，进行PCR分析PCR产物应用焦磷酸测序PSQ96 MA测序仪进行SNP检测，用质谱法进行及等位方基因频率分析。

METHODS:The gene of human ET1 was synthesized according to the preferential codons of E. coli, cloned into the EcoRI and SalI sites of vector pThioHisA. The recombinant plasmid pThioHisA-ET1 was constructed , sequenced and transformed into E.coli TOP10. Induced and expressed fusion protein were identified and analysed by 12% SDS-PAGE and densitometry analyses. After the elution, denature and renature, the fusion protein Thioredoxin-ET1 was obtained by ProBondTM chromatogragraphy. The purity of Thioredoxin-ET1 was detected by HPLC. Inoculate Thioredoxin-ET1 once per mouse every 2 weeks in 25, 50 and 100μg separately on 3 groups for 4 times. 10 days after last inoculation, we obtained venipuncture blood.

根据人ET1的多肽序列合成ET1基因，将其插入到pThioHisA的EcoRI和 SalI位点，重组质粒pThioHisA-ET1进行酶切鉴定及序列测定验证后转化TOP10，IPTG诱导的重组菌经SDS-PAGE检测融合蛋白Thioredoxin-ET1的表达量；表达的融合蛋白用ProBond亲合层析纯化并经HPLC鉴测其纯度；每只小鼠按25、50、100ug/次剂量的Thioredoxin-ET1每两周免疫一次，共4次，最后一次免疫10d后制备抗血清，经Western blot和ELISA检测证明Thioredoxin-ET1融合蛋白具有ET1免疫反应原性。

In order to understand the expressed genes, construct the known gene expressed profile, study the tissue-specific genes and the common genes from pig and chicken; In order to do primary researches for comparative genomics, functional genomics, QTLs mapping, animal molecular breeding and so on, we have constructed 3 cDNA libraries of Xiang Pig muscle tissue, Silkie Fowl muscle tissue and liver tissue, sequenced a large scale ESTs and analyzed their bioinformation from the ESTs of Silkie Fowl muscle tissue and liver tissue in our study.

为全面了解肌肉组织和肝脏组织的基因表达类型，构建猪、鸡肌肉组织和肝脏组织的功能基因表达谱，研究肌肉组织和肝脏组织的特异表达基因及这两种组织的共同基因；为比较基因组学的研究、功能基因组学的研究、QTLs定位等的动物分子育种做前期基础科研工作，本研究构建了香猪肌肉组织、丝毛乌骨鸡肌肉组织和肝脏组织的3个cDNA文厍，并对丝毛乌骨鸡的肌肉组织和肝脏组织进行了大规模的ESTs测序及其生物信息学的初步研究。

Duck IFN-γ gene was amplified from total RNA extracted directionally from ma duck splenocyte by reverse transcription-polymerase chain reaction, then was cloned and sequenced.

应用RT-PCR技术，直接从成年麻鸭脾淋巴细胞提取的总RNA中扩增出麻鸭γ-干扰素基因，并克隆、测序。

Porcine mature protein IL-18 gene was amplified by RT-PCR from porcine splenocyte total RNA extracts and then was cloned and sequenced.

采集6月龄河南良杂猪的脾脏，分离淋巴细胞后直接提取总RNA，进行反转录－聚合酶链反应扩增，扩增产物进行T-A克隆、测序，获得了河南良杂猪IL-18基因成熟蛋白序列。

Methods Routine method was used to isolate E.coli, antibiotics suscept ibility was tested by the disk diffusion method; class 1 integron was detected by PCR assay; PCR products were sequenced and analyzed.

常规方法分离大肠埃希菌；纸片扩散法对10种抗生素进行耐药性监测和分析；PCR鉴定第1类整合子阳性株；PCR产物测序并对结果进行分析。

As part of an international effort to completely sequence the rice genome, we have produced a fine bacterial artificial chromosome-based physical map of the Oryza sativa japonica Nipponbare chromosome 4 through an integration of 114 sequenced BAC clones from a taxonomically related subspecies O.

国际水稻基因组测序计划(International Rice Genome Sequencing Project)采用以物理图为基础的随机测序战略，作为该计划的一部分，中国科学院国家基因中心承担了水稻第四号染色体精确序列的测定任务。

We amied the BCP sequence and the preS2 gene sequence of HBV separately as the target region, according to HBV DNA sequence of Chinese strain, synthesized the set of specific primers, amplified the sequence by PCR method from the serum of patients with CHI, verified by restriction analysis, subcloned into pGEM Teasy vectors, employed polyacrylamide gel electrophoresis to display the deletion mutation and selected the clones with differential length to be sequenced.

本研究应用PCR技术及其他分子生物学技术，分别以HBV转录调控序列BCP以及表面蛋白编码序列pre S2为靶基因，以中国株HBV基因序列为依据，设计特异性多聚酶链反应引物，分别自43例、51例慢性HBV感染患者血清中扩增出目的片段，构建质粒Teasy-BCP和Teasy-pre S2，酶切鉴定后，采用聚丙烯酰胺凝胶电泳技术展示缺失突变，再经DNA测序以了解在慢性乙型肝炎患者体内的病毒变异情况。

A set of spectific primers was syn thesized according to HBV DNA sequence of Chinese strain, the whole X region was amplified by PCR method from the serum of 9 patients with chronic HBV infection , and then the PCR products were subcloned into pGEM Teasy vectors. Clones were randomly selected to be sequenced. Comparison of the cloned sequence was made to find the difference. After being compared, each sequence of selected clones is o f difference. The point mutation scattered through X region. Deletion mutations were detected in 19 clones of 37(51.4%), which caused different carboxyl endings of X protein. There is a hot region (after 123 aa code) where deletion mutation frequently happens.

以中国株HBV基因序列为依据，设计特异性多聚酶链反应引物，自9例慢性HBV感染患者血清中扩增HBV X基因，克隆入pGEM Teasy质粒，随机挑选克隆进行D NA测序以确定病毒的变异程度。37例测序结果提示来源于不同患者HBV X基因序列高度保守，但每个序列均不一致。X区除了存在广泛的碱基点替换突变外，序列的缺失突变占测序克隆总数的51.4％（19／37）；氨基酸缺失及移框突变多发生于123位氨基酸残基之后，可导致X蛋白多种羧基端形式。

Methods The resistance of Salmonella isolates were detected by drug susceptibility tests with KirbyBauer diffusion method. PCR was designed to detect tetA, tetB, tetC, tetD, tetE, tetG and tetK. The amplified products were sequenced and alignment analysis was performed.

用KB法测定分离株对四环素等21种抗生素的耐药情况，用PCR方法检测四环素耐药基因tetA、tetB、tetC、tetD、tetE、tetG及tetK，并对扩增产物进行测序。

"Second Life is remarkably easy to work with, and is very popular,"

"第二次生命是显着容易的工作，并且很受欢迎，"

For example, we usually assume that materials are homogeneous and isotropic and free of internal defects or flaws.

为了得到适合有限元分析的模型，我们必须经过如图2所示的简化步骤。