查询词典 sampling cell

The results of transient transfection showed that the recombinant plasmid could be lowly expressed in the murine macrophage cell line RAW264.7 and murine melanoma cell line B16, both of which can endogenously express Tim-3, but not in the African green monkey kidney cell line COS-7 in which no endogenous Tim-3 could be detected.

结果 pGL3-Tim-3P2经PCR、双酶切及DNA测序分析鉴定正确无误；pGL3-Tim-3P2转染RAW264.7和B16细胞（两种细胞均可表达内源性Tim-3）24 h和48h，双荧光素酶活性检测显示其启动子相对活性约为pGL3-Basic空载体的2倍；而pGL3-Tim-3P2转染不表达内源性Tim-3的COS-7细胞后检测不到荧光素酶活性。

Finally the pathological change implicates the lung section bronchial tube pulmonary alveolus fills in the cleaning solution cd3 t cell obviously to be lower than does not have the lung section group which the pathological change implicates, cd4 t cell, cd4/cd8, the nk cell obviously to be lower than the opposite side lung section group which the pathological change has not implicated.

结果 病变累及的肺段支气管肺泡灌洗液中cd3t细胞明显低于没有病变累及的肺段组，cd4t细胞、cd4/cd8、nk细胞明显低于病变没有累及的对侧肺段组。

In order to make the scan data of cell or line detectors include corresponding space information and enhance the resolvability of infrared image, the reconstruction strategy of infrared image was proposed. It was based on the mechanism of variable row frequency sampling under cone scan.

为了使一些单元或线列探测器获得的扫描数据含有相应的空间信息，进而提高获得红外热像的分辨率，提出了圆锥扫描机制下基于变行频采样机理的红外图像完整重建策略。

To produce medicine serum of Liu-Shen-Wan with different dose, different time of pouring medicine, different time of blood sampling.2. To measure content of arsenic in serum with atomic absorption method, to qualitative analysis toadfish in medicine serum of Liu-Shen-Wan with thin layer chromatography.3. MTT and trypan blue assay detects the growth inhibition effect of Liu-Shen-Wan on HL-60 cells and to make certain the best scheme of making method of medicine serum of Liu-Shen-Wan.4. Gimsa dying assay observes morphology changes of apoptosis .In situ end-labeling method and flow cell machine quantificationally probes apoptosis phenomena.

1 分不同的给药剂量、给药天数和不同的采血时间制备含药血清。2 用原子吸收法测定血清砷含量；薄层色谱法定性检测六神丸含药血清中蟾酥成分。3 用MTT法、胎盘蓝染色法观察六神丸对HL-60细胞的增殖抑制作用，确定最佳血清制备方案。4 用吉姆萨染色法观察细胞凋亡的形态学变化，流式细胞仪、原位末端标记法定量检测细胞凋亡程度。

Results: Amang 128 patients, 56 cases were diagnosed as pulmonary carcinoma (43.75%), of which 30 cases were squamous carcinoma (53.57%), 22 cases were adenocarcinoma (39.29%), 4 cases were small cell undifferentiated carcinoma (7.14%); 37 cases were bronchial pneumonia (28.91%), in which 30 cases were given brush sampling, bacterial culture of BALF, the detectable rate of pathogenic bacteria were 50%(15 cases) and 40%(12eases) respectively; 12 eases were pulmonary tuberculosis (9.38%), of which 3 eases (25%) had concurrent endobronchial tuberculosis, smear of brush biopsy and BALF found Mycobacterium tuberculosis were 11 eases (91.67%) and 5 eases (41.67%) respectively, 2 eases were diagnosed as caseous tubereulous granuloma (16.67%); 2 eases were idiopathic pulmonary fibrosis (1.56%), lease was lymphoma of long.

结果：128例患者确诊为肺癌56例(43.75%)，其中鳞癌30例(53.57%)，腺癌22例(39.29%)，小细胞癌4例(7.14%)；支气管肺部炎症病变37例(28.91%)，其中30例经防污毛刷采样、支气管肺泡灌洗液行细菌学培养，病原菌的检出率分别为50%（15例）和40%（12例）；肺结核12例(9.38%)，其中合并支气管内膜结核3例(25%)，刷检涂片和支气管肺泡灌洗液找到结核杆菌分别为11例(91.67%)和5例(41.67%)，病理确诊为结核性乾酪样肉芽肿2例(16.67%)；特发性肺间质纤维化2例(1.56%)，肺淋巴瘤1例(0.78%)。

Methods:The randomly group sampling,parallet comparison and dilamination methods are used in this study.166 cases (10 falling off) was observed on giving priority to diferent treatment of yiqi yangyin,wenbu spleen and kindey,qingre jiedu and evaluate the change of different quantity arrangement of CD4~+T lymphocyte cell of different treatment of TCM on HIV asymptomatic carriers.

采用随机整群抽样、平行对照、分层研究的方法，分别以益气养阴法、温补脾肾法和清热解毒法为主对166例(脱落10例)感染者进行干预，观察中医不同治法对感染者不同层次CD4~+T淋巴细胞的变化。

The closed-cell aluminum foam was fabricated by direct foaming in melt. Through sampling aluminum foam in different stages the cross-section of them were observed and analyzed. It was explained how TiH_2 was decomposed after entered into the melt, how initial bubbles were formed and what was the state that generated bubbles and undecomposed TiH_2 lay in.

以熔体直接发泡法制备闭孔泡沫铝材实验为基础，通过获得不同实验阶段的泡沫铝样品，以及对实验样品切面或断面进行观察和分析，描述了在熔体发泡法制造泡沫铝过程中TiH2加入熔体后的分解过程，原始气泡的形成方式以及产生的气泡和未分解TiH2的存在状态；解释了气泡进一步长大的原因和未分解的TiH2如何释放气体；表述了气泡的合并和无泡层的形成。

The studies exploring the relationship between HPV infection and laryngealcarcinoma began in 1980s,since then,almost all the research was aimed at theinvestigation of the HPV prevalence in the biopsies,due to differences in thechoice of DNA detection method,cell sampling and the geogen,the prevalenceestimates of HPV have varied accordingly,and led to two different points about therelationship between HPV infection and laryngeal carcinoma:some researchersdemonstrated that a higher HPV prevalence could be found in laryngeal carcinomas(from 50% to 90%)than in benign laryngeal lesions,suggesting a relationshipbetween HPV infection and laryngeal carcinoma;but the other researchers foundthat the isolation of HPV is no greater in the laryngeal cancers(from 5% to 43.3%)than in the normal larynx or benign laryngeal lesions,thereby questioning itsctiological significance,so,the role of HPV in the development of laryngealcarcinomas still remains unclear.

有关HPV与喉癌的研究始于80年代，但20多年来研究模式始终停留在对临床标本进行检测的水平上，由于各家采用的检测手段敏感性不同、标本数量以及来源地域也存在差异，使各家学者对喉癌中HPV阳性率的报道极不一致，并由此导致了两种截然不同的观点：一部分学者报告的喉癌标本中HPV阳性率高达50%~90%，并显著高于喉良性肿瘤，认为HPV与喉癌有关；而另有不少学者检测的样本中，阳性率（5%~43.3%）并不比正常喉粘膜及良性肿瘤高，认为HPV与喉癌无关，因此，HPV到底是不是喉癌的致癌因素目前仍无定论。

In part 5, writing the procedure to test the MTF of Infrared detector, protracting the coordinate of MTF_Frequency and the single cell Infrared detector curve of MTF_Frequency; sampling the data of the second-generation Infrared detector are the main jobs.

第五章编写了IRFPA MTF测试过程软件，介绍了288×4红外焦平面阵列探测器的信号采集。绘制了刀口响应曲线及其微分曲线和系统的MTF曲线并对它们进行了分析。

Its reasonable for us to propose the following hypothesis: in the early period CAN, MMPs/TIMPs system is a pivotal figure for mononuclear cell infiltration and vascular smooth muscle cell proliferation and migration through its effects on matrix remodeling, thusinfluences the late survival of allograft.

我们根据这些研究结果提出以下假说：MMPs/TIMPs系统在慢性移植肾病早期通过促进细胞外基质重新排列而促进单个核细胞浸润和血管平滑肌细胞移行，从而影响移植物的长期存活。

"Second Life is remarkably easy to work with, and is very popular,"

"第二次生命是显着容易的工作，并且很受欢迎，"

For example, we usually assume that materials are homogeneous and isotropic and free of internal defects or flaws.

为了得到适合有限元分析的模型，我们必须经过如图2所示的简化步骤。