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Japonicum was firstly PCR amplified from S. japonicum cercaria cDNA library. Its recombinant prokaryotic and eukaryotic expression plasmids were successfully constructed and its nucleotide sequence was determined. It was firstly expressed in E. coli with MW of about 17 ku.

中山大学基础医学院病原生物学教研室；中山大学基础医学院病原生物学教研室；中山大学基础医学院病原生物学教研室；中山大学基础医学院病原生物学教研室；中山大学基础医学院病原生物学教研室广东广州510189；广州医学院病原生物学教研室；广东广州510180；广东广州510189；广东广州510189；广东广州510189；广东广州510189

Results showed that specific SIEA26~28 ku scFv with high level expression was achieved. The corresponding gene, ribosomal protein S4 was obtained by initially screening the cercaria cDNA library with the specific SIEA26~28 ku scFv. The obtaining of specific SIEA26~28 ku scFv lays the foundation for further screening and identification of the coding genes of SIEA26~28 ku, the natural molecular candidate vaccine against schistosomiasis.

结果显示，所获得的SIEA26~28ku特异性scFv，表达量高，采用该探针初步筛选出相关基因核糖体蛋白S4SIEA26~28ku特异性scFv的获得，为进一步筛选、分析鉴定抗日本血吸虫病天然分子候选疫苗SIEA26~28ku的编码基因奠定了基础。

A method based on polymerase chain reaction for screening cDNA library is described.

建立了一种以PCR为基础的快速筛选cDNA文库的方法。

This chakra is one's master library of spiritual knowledge.

该脉轮是你主要的灵性知识库。

In view of the complexity of mechanical products, the functional characteristic model and the knowledge library of function parameters were established.

在对机械系统的组成进行分析的基础上，建立了功能特征模型和功能参数知识库，运用变功能参数操作，实现了产品功能的变异和扩展以及结构的重构，从而达到产品创新设计的目的。

In view of the complexity of the function of mechanical products, the functional characteristic model and the knowledge library of function parameters were established. Through the function parameter mutation operation, then function and structure of products were modified and generated.

在对机械系统组成进行分析的基础上，建立了功能特征扩展和功能参数知识库模型，运用变功能参数操作，实现了产品功能的组合、变异和扩展，并使得产品结构相应的发生改变和重构，从而达到产品创新设计的目的。

You'll probably find a similar range of books to the library sale ones; charity stores may sell books in "nearly new" conditions, whereas school fetes and sales are likely to have more battered volumes.

也许你会发现这些书的范围和在图书馆售书日提供的差不多。非营利商店的书籍几乎是新的，而校庆时打折减价的书则多有破损。

I don't like the book borrow from others or library.the borrowed book seems charmless to me ,just a book bought myself can satisfy me.it can call an occupy desire, buying some books and affixing a seal to every book would be the most wonderful time for me.

我不喜欢向别人或图书馆借书。借来的书，在我好像过不来瘾似的，必要是自己买的才满足。这也可谓是一种占有的欲望。买到了几册新书，一册一册在加盖藏书印记，我最感到快悦的是这时候。

Charwoman: You mean the library?

你是说书房吗？

I want to check this book out of the library.

我想把这本书借出图书馆。不适合

"Second Life is remarkably easy to work with, and is very popular,"

"第二次生命是显着容易的工作，并且很受欢迎，"

For example, we usually assume that materials are homogeneous and isotropic and free of internal defects or flaws.

为了得到适合有限元分析的模型，我们必须经过如图2所示的简化步骤。