查询词典 mut

Methods Amplify IL-6 (23)-PE40 gene from the previously constructed recombinant plasmid pKK-IL-6(23)-PE40 by PCR, and insert to the SnaB Ⅰand NotⅠ sites of vector pPIC9. Transform the constructed recombinant plasmid pPIC0-SN to P. pastoris GS115 by electroporation, screen Mut-recombinants for expression under induction of methanol.

采用PCR技术，将目的基因IL-6(23)-PE40插入到pPIC9载体中SnaBⅠ与NotⅠ位点，电转化至巴斯德毕赤酵母GS115中，筛选Mut-型重组酵母；甲醇诱导表达，体外细胞试验检测其细胞毒性。

Therefore, it is important to ask whether both Oct-2 and NF-κB participate the MEK/ERK dependent G-CSF expression. G-CSFp-(-289~+25)-Luc, CSF-box-deleted G-CSFp-(-145~+25)-Luc, or octamer-mutated Oct-mut-G-CSFp-(-289~+25)-Luc reporter plasmids were used to investigate the importance of CSF-box and octamer in response to LPS.

在G-CSF的启动子上有两段已被发现对其活性相当重要的序列：CSF-box及octamer，根据过去研究及本实验室未发表的实验结果，此两段序列分别由NF-κB以及Oct-2所结合；於是我们推测MEK/ERK讯息传递路径的下游可能透过NF-κB及Oct-2来调控G-CSF表现。

All Mut+and Muts colones were induced respectively with methanol to secrete interesting peptide in shake-flask cultures for four days. After dialysis and lyophilization, the supernate of cultures were identified by SDS-PAGE and Western blotting to find the genetically engineered Pichia pastoris which expressed interesting peptide.

在摇瓶中分别用甲醇诱导Mut~+和Mut~s型转化子表达蛋白4d，取培养产物冻干浓缩，进行SDS-PAGE和Western blotting，分析蛋白的含量及纯度，筛选出能特异表达目的蛋白的基因工程菌。

Jaggers seems to be an important andpowerful man:hordes of people wait outside his office,mut.tering his name among themselves.

贾格斯似乎是个重要的、有影响的人物：。A_，frj成群结队地等在他的办公室外，不停地谈论着他。

This event was caused by the solar activities such as increases of the sunspot numbers, occurrence of a 3b optical flare at 22 h46 mUT on March 22,1991 and so on.

这次磁暴是由磁暴前太阳黑子相对数增多、3月22日22h46mUT有3b级耀斑爆发等一系列太阳活动所引起的

This block statue is one of four from the Temple of Mut at Karnak.

此 b 4座雕像是一个从庙在卡纳克物。

Mut, Just look at what you've done!

看看你都做了些什么！

They must have an undertanding of the needs of society,and they mut also learn how politics works.

他们必须了解社会需求，同时必须清楚政治是如何运作的。

He's blind, not mut e - so simple.

他是瞎子，不是哑巴。

Last mut not least, we must thank our host for his hospitality

最後但并非最不重要的一点是，我们得感谢主人的热情好客

Chinese traditional virtue is humility and wariness,the compliment and praise to the others should be declination

中国人的传统美德是谦虚谨慎，对别人的恭维和夸奖应是推辞。如

We bought this house on the never-never.

我们以分期付款的方式买下这座房子。