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The ELISA titre was 1:2000.By cell fusion, 46 hybridoma cell lines were screened,and 10 lines were cloned with limited dilution method.16 lines secreting anti-bFGF monoclonal antibody were been developed, and 2 lines targeted fusion protein. Sensitive ELISA and dot-ELISA for bFGF was developed with this mAb. The detection limit of them were 0.1 ng/well and 0.5 ng/well. The expression level of anti-bFGF mAb by different rebuilt engineering cells were identified by western blot and to direct rebuild recombiment engineering cell. The dose and character of anti-bFGF mAb inhibiting bFGF biology activity were searched by 3T3 cell line. Searching 20 tissue of liver cancer, liver cancer cell lines and general tissue of liver, finding bFGF were highly expressed in tissues of liver cancer and liver cancer cell lines. Affinity chromatography purifying bFGF was set up by mAb binging CNBr-pepharose 4B, and the purification was 95%. We found that the titer of anti-bFGF antibody was very high in serum of neuropathic amyotrophia.

应用细胞融合制备46株杂交瘤，对其中10株进行克隆化，获得bFGF特异单抗16株，2株针对融合蛋白；应用该单抗建立了0.1ng/孔灵敏度的ELISA，0.5ng/孔敏度的斑点ELISA；用Western-blotting鉴别了经改造不同工程菌蛋白表达，指导重组工程菌改造；用3T3细胞培养研究了单抗抑制bFGF生物学活性的剂量和特点；合作研究了20例肝癌、肝癌细胞株和正常肝组织，发现前者bFGF高表达；应用单抗偶联CNBr-sepharose 4B建立了小量免疫亲和层析纯化bFGF，纯度达到95%；发现神经性肌萎缩患者血清中含有高滴度的bFGF抗体，已有10多家单位引用单抗或进行合作。

For years, Mr. Zhou has been engaged in the research of aseismic Engineering, and the exploitation and development of Earthquake Engineering.

多年来一直从事工程抗震研究工作，致力于地震工程学的开拓和发展。

The application about neural networks used in process control is studied in light of auctorial engineering practice. Neural networks for process identification and control has been used in CAE2000 (a computer aided engineering software project) and the LN2000 which are developed by ourselves.

根据作者的工程实践，对神经网络在过程控制工程中的应用问题作了应用研究，包括在自主开发的控制系统计算机辅助设计CAE2000系统中的应用研究，以及在自主开发的分散控制系统LN2000中的应用研究。

The presterssed anchor cable frame combined with backwall, drainage is necessary due to longtime sability and permanent utilization.Firstly,according the study of engineering of geology situation of the high slope, the mechanism of deformation failure and the major hazard of the high slope of PanMei is analysed comprehensively.And the whole slope is divided into two different geology enginnering areas:Western slope(1160 section plane -1240 section plane)and Eastern slope(1240 section plane -1320 section plane). The result of 3D numerical simulation is the same as geology judgement. Later,slope cut (1:0.75) is implemented based on the engineering comparison and criterion.Meanwhile, Limit equilibrium method , Monte Carlo Probabilistic slope stability the stability , Sensitivity analysis and Finent element method of for assessing the cutted slope stability is carried out. The result indicates that Western slope is instable, Eastern slope is relatively stable.So much attention should be paid to Western slope.Different reinforcement measures in different areas are given according to its stability and meet economical request. Gravity wall is introduced in accordance with landform in order to prevent small falling at the foot of the high slope ,which will be a threat to the high slope stability. Handling measure is also applied to Eastern slope. At last,FLAC3D numerical analysis shows the feasibility and reasonableness of the handling measure of the high slope.

论文首先研究了边坡的工程地质条件，全面分析边坡的变形破坏机制和边坡的主要地质灾害，将边坡分为西段不稳定边坡区(1160剖面～1240剖面)和东段相对稳定边坡区(1240剖面～1320剖面)，通过三维数值模拟再现了边坡在自重作用下的破坏机制和过程；在此基础上通过工程类比和规范要求，确定按照1：0.75进行放坡处理，并分析了放坡后边坡的稳定性状况，分别通过极限平衡法、蒙特卡洛法概率分析、参数敏感性分析和有限元稳定性计算等多种方法对边坡在各种工况下的进行稳定性研究，计算结果表明，西段边坡较之东段边坡稳定情况差，是边坡支护的关键之处，而东段边坡相对是稳定的；边坡的综合治理措施选用的是框架锚索并结合挡土墙和排水工程，治理设计根据不同区段的稳定性情况采用不同的支护结构，做到了经济合理的要求；在边坡的坡脚设置重力式挡土墙，防治边坡小规模的崩塌，会对上部的锚索支护护造成不利影响，进而影响边坡的稳定；东段边坡的填方区也给出了治理对策；最后通过三维显示拉各朗日有限差分程序(FLAC3D)模拟了高边坡放坡和支护效果，论证了治理措施的有效性和合理性。

The bearing capacity of foundation soil and batholith is one of the most important questions for discussion in the field of geotechnical engineering ;md foundation engineering.

地基及岩基承载力问题是岩土工程及基础工程领域最重要的课题之一。

As an important branch of biochemical engineering, bioreaction engineering focuses on resolving the technical problems relating to biochemical reactions in the course of transforming biotechnology from laboratory results to industrial production in large scale.

生物技术作为具有巨大发展潜力的高科技之一，不断取得新的研究成果，在医药、食品、环境、卫生、能源等诸多领域发挥的重要作用日益受到广泛关注。

Based on the above research, the article discusses the process using ASP and ADO technique to manage and update database; studies the setting of purview to assure the security of system; discusses the function of layercontrol, eagle-eye navigation, search of information and etc.; discusses the function of statistic and analysis by the process doing the caky statistic chart of roadbed disease; analyzes the doing of distributing special map of disease and engineering with the character of rail-track engineering GIS.

在以上总体研究的基础上，讨论了利用ASP和ADO技术管理和更新远程铁路工务数据库的实现过程；为了保证系统使用的安全性，研究了对用户使用权限的设置；讨论了图层控制、鹰眼导航等地图操作功能以及信息查询功能的实现过程；以路基病害分析的饼状统计图的制作过程为例讨论了统计分析的功能；针对铁路工务地理信息系统的特点，分析了病害和维修工程分布专题图的制作。

After finishing her BTech in Architectural Engineering from NIT- Calicut in 2000, Monika studied for Master's Degree in "IT in Building Science", a two year masters program from IIIT-Hyderabad in 2003, which enables architects with software engineering expertise to understand and write their own software tools.

Monika Prabhu 2000年在印度的 NIT- Calicut大学建筑工程学院获得学士学位，之后在印度的IIIT-Hyderabad大学深造，并于2003年获得IT建筑科学硕士学位，该课程为两年，旨在让具有工程师背景的建筑师理解并编写软件程序作为设计的工具。

The study at the undergraduate stage will provide students with strong backgrounds in theoretical fundamentals and systematic expertise of materials science and engineering, help students learn about the trend of development in this field, master necessary experimental and computational methods, skills, and have a good command of a foreign language, educate students to develop engineering problem solving skills or enable them to develop new materials, products, processes, and equipment, and capacitate them to sort out problems in practical applications by methods of analyzing chemical composition and structure of materials, controlling material manufacturing process and quality, and testing and analyzing material properties, and by techniques of synthesizing materials and processing material products.

学生通过本科阶段的学习，牢固掌握材料科学与工程学科理论基础和系统的专门知识；了解本领域的发展动向；掌握必要的实验、计算方法和技术；精通一门外国语；具有解决工程问题或从事新材料、新产品、新工艺、新设备的开发能力；能运用材料化学成份和组织结构的分析方法、材料的制造过程和质量控制方法、材料性能检测和分析方法、材料的改性技术、材料制品的加工工艺和技术等解决实际应用问题。

Thus the scope of Concurring Engineering enablers is extended. The DFRP theory is now registered in Engineering Index and generally accepted.

目前，该理论已经得到许多研究人员的认可，相关研究论文和成果也已获得美国工程索引的检索。

When this condition occurs, inbound replication with the source partner is stopped on the destination domain controller and event ID 2042 is logged in the Directory Services event log.

计算机密码学是研究计算机信息加密、解密及其变换的科学，是数学和计算机的交义学科，也是一门新兴的学科。

Instructions: click on the thumbnails to see a larger image, then use the left-right arrow keys to scroll through the slideshow.

使用说明：滑鼠点在小图上即可放大观赏。开启后键盘左右键可用来换照片。