查询词典 labeling

Result: the regions with strong to very stong labeling by the GDNF probes were as follows: the caudate-putamen, the habenular nucleuse, and some areas of the brainstem and spinal cord, the trigeminal ganglion, the dorsal root nerve ganglion.

本实验利用原位杂交技术结合RT-PCR技术观察了GDNF从E16～成年大鼠神经系统内的分布；为了更进一步研究GDNF在神经发育中的作用与作用机制，本实验还利用基因克隆技术克隆了人GDNF基因并进行了原核表达GDNF蛋白的研究。

The heterogenic C57BL/6J splenocytes disappeared at 1 day after transfusion, but the syngeneic BALB/c splenocytes could survive for a long time in the peripheral blood of BALB/c mice. The fluorescence intensity was not decreased in two kinds of splenocytes. The results demonstrated that CFSE-labeling with difference concentration is reliable, stable and convenient for spleen lymphocytes tracing in vivo.

异基因的C57BL/6J脾细胞在输注1 d后就快速消失，而同基因BALB/c脾细胞能够在BALB/c小鼠外周血中长期存在，但是两种细胞荧光强度均未见降低，说明荧光染料CFSE差异浓度标记活细胞进行体内示踪是一种稳定的示踪方法。

Methods:Animal models of 25 adult mixed breed dogs were established by transverse osteotomy at bilateral iliopectineal crest,which were fixed with ATMFS anterior column connector and steel plate respectively. The animals were examined at 2,4,6,8,and 12 weeks by X-ray,macropathological observation and biomechanical test. Tetracin fluorescent labeling,HE staining,Masson triad colour staining,in situ hybridization and immunohistochemical method were performed and histological image analysis were applied.

建立犬双侧骨盆弓状线骨折的动物模型，分别采用ATMFS前柱固定器和5孔重建钢板内固定，于术后2、4、6、8、12周分别行影像学检查、大体观察及生物力学测试；采用四环素荧光标记法、HE染色、Masson三色染色法、原位杂交法、免疫组织化学法进行组织学观察并图像分析；采用RT-PCR法检测骨折端不同时期骨钙蛋白和核心转录因子mRNA相对表达量的变化。

It can handle a wide range of multi-substrate packaging and labeling materials, aided by a servo-driven infeed, servo drives on all print units, as well as a mid-feed pacer and die-cutter units.

它可以处理广泛的多基质包装和标签材料，由资助伺服驱动进给，在所有印刷单位的伺服驱动器，以及一个中期饲料起搏器和模切单元。

Results Microstructure of callus showed that the area of mature trabecular in callus of OPFM group was smaller than that of CFM group, and was thinner with a wide intertrabecular distance. The dynamic parameters demonstrated that the fluorescent labeling percentage on the trabecular surface of OPFM group was lower than that of CFM group, but bone mineral apposition rate was higher.

结果 ①骨痂组织骨组织形态计量学结果发现：OPFM组成熟小梁骨占骨痂面积比CFM组小，且小梁骨厚度变薄、小梁骨间距较宽；同时骨小梁动力学参数显示：OPFM组骨小梁表面荧光标记百分比较CFM组低，而骨矿化沉积率却较高。

We introduced improved primary mixed glial culture and different-attachment method to isolate and purify the OPCs, the cells were proliferated in serum-free medium, flow cytometry and immunohischemistry methods were employed to estimate the purity of cultured OPCs. Their abilities of differentiation and expression of trophic factors were identified by RT-PCR and immunostaining. Several methods including TUNEL and MTT were adopted to estimate the protective effects of conditioned culture medium from oligodendrocyte lineage cells on the primary cultured cerebellar granule neurons. Intravitreal transplant of OPCs, combined with retrograde fluorescent labeling the superior colliculus and intraorbital optic nerve transection, were used to investigate the protective effects of OPCs on the axotomized RGCs in vivo. Intravitreal transplantof OPCs or NSCs on the newborn rats, and retinal transplant of OPCs on the young rats were performed, to observe the myelin formation in the retina at different stages after cellular transplantation. Optic nerve transection was carried out on some rats with myelinated retinae, to study the influence of myelination on the injuried RGCs.

为此，本研究采用改良的胶质细胞混合培养与差速贴壁方法获得大鼠OPCs，使用无血清培养基进行扩增、培养，用免疫组织化学和流式细胞技术对培养细胞的纯度进行鉴定，对少突胶质系细胞表达部分营养因子的情况进行检测；采用TUNEL、MTT等方法对少突胶质系细胞条件培养基对原代培养小脑颗粒神经元的保护作用进行检测；将OPCs移植入成年SD大鼠玻璃体内，利用上丘逆行荧光标记技术，观察眼内移植的OPCs对眶内视神经切断时的视网膜神经节细胞的保护作用及其持续时间；将OPCs或NSCs移植入新生和幼年SD大鼠玻璃体或视网膜内，观察不同时期视网膜内髓鞘形成与分布特点，分析髓鞘的超微结构，并观察眼内髓鞘形成对损伤神经节细胞的保护作用。

Keywords: diketoreductase,isomeride,separation and purification,statins chiral side-chain expression, photoaffinity labeling probe

通过DEAE-Sepharose离子交换柱及Sephadex分子筛柱纯化后，酶的纯度达到电泳纯，并通过正相硅胶柱和反相C-18柱分离纯化其反应产物。

In situ end-labeling was used in the light of instruction of Roche apoptosis kit to detect the apoptotic endothelial ceils. The ceils with brown karyon stained were deemed to be apoptotic cell under Olympus light microscope.

采用原位末端标记法，参照罗氏公司细胞凋亡检测试剂盒说明书检测治疗前后内皮细胞凋亡情况，OLYMPUS光学显微镜下观察，细胞核被染成红棕色的细胞视为凋亡细胞。

When you only need to detach from the backing sheet, just one click, you can stick to a variety of substrate surface, or use the labeller an automatic labeling on the production line.

应用时只需从底纸上剥离，轻轻一按，即可贴到各种基材的表面，也可使用贴标机在生产线上自动贴标。

Methods:Myocyte apoptosis in patients with sudden death caused by AMI and in healthy subjects who died in traffic accident were studied by means of TdT-mediated dUTP Nick End Labeling,DNA laddering on agarose gel electrophoresis and electron microscopy.

用末端原位标记法、DNA电泳和电镜3种方法检测急性心肌梗死猝死患者和心脏正常的车祸死亡者心肌细胞的凋亡。

"Second Life is remarkably easy to work with, and is very popular,"

"第二次生命是显着容易的工作，并且很受欢迎，"

For example, we usually assume that materials are homogeneous and isotropic and free of internal defects or flaws.

为了得到适合有限元分析的模型，我们必须经过如图2所示的简化步骤。