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Methods Enzyme digestion was used to isolate single ventricular myocyte and whole cell patch clamp technique was used to record potassium current.

方法酶解法分离豚鼠单个心室肌细胞，应用全细胞膜片钳技术记录延胡索乙素对豚鼠单个心室肌细胞钾电流的影响。

To isolate and clone the tumor suppressor gene on chromosomal region 7q32 that corelated with the occurrence of human NPC,we detected the genotype of polymorphic microsatellite markers on 7q32 in 24 nasopharyngeal carcinoma biopsies and matched normal lymphocyte DNA.

为了分离和克隆定位于7q32染色体区域的与鼻咽癌发病有关的抑瘤基因，检测了鼻咽癌活检组织及配对外周血标本中7q32微卫星DNA多态性位点的基因型，发现在7q32区域存在30%左右的杂合性丢失。

They were divided into three groups. The first group, included most of highly virulent isolates from different corn-growing areas, exhibited high similarity in RAPD patterns and completely identical ITS sequence except for one isolate.

第一组为优势组，多数菌株间相似性高，在ITS区域具有完全相同的DNA序列，包含来自各发病区的大多数致病菌株，是引起玉米弯孢菌叶斑病的优势类群。

At the same time, the fire prevention system design, will survey, the monitoring and the manually controlled alarm system and the fire broadcast, Fang the curtain gate and the fire pump unites, realization fire prevention linkage, when will have the fire, the survey, the monitoring and the hand-controller system will send out the fire signal, will have various system to send out the warning, will carry on the fire protection to isolate and to start the automatic fire fighting system, while will disperse personnel's to carry on the automatic fire fighting, the control, the elimination fire hidden danger, will safeguard in this building the personnel, the property safety.

同时，消防系统的设计，将探测、监控和手控报警系统与消防广播，房火卷帘门和消防水泵相联，实现消防联动，当有火灾时，探测、监控和手控系统发出火灾信号，有各联动装置发出警报，进行防火隔离和启动自动灭火系统，在疏散人员的同时进行自动灭火，控制、消除火灾隐患，保障该楼内人员、财产的安全。

This advantage will increase demand from the result of the fuel cell, which is a complicated system for the lost of determinate mathematical model. The other advantage is that the development time of the DMFC controller can be tremendously decreased by using the RTW and Embedded Target C2000 tools of the MATLAB to generate the control code for the RCP controller. Meanwhile, in addition to implementing a TI emulation board F2808eZdsp to be the controller, an interfacing circuit, functioning to isolate electric connection and driving actuators between the board and OSS, is designed and fabricated in this research.

关於直接甲醇燃料电池，由於未见完整且可用於解析性控制器设计之直接甲醇燃料电池的动态模型，必然需要较复杂之控制系统，故本研究将结合MATLAB的控制器快速原型方法应用於直接甲醇燃料电池机电系统之控制，不仅具有控制器原型快速产生的优点，同时可以结合MATLAB中有关各式控制器设计的资源，设计出更优异的控制器。

Objective To isolate,identify and purify the allergens in egg.Methods The albumen was extracted from the egg and the albumen from the vitellus.The total protein of the egg was isolated by SDS-PAGE.The allergens of egg were identified by Westernblotting.

分别提取鸡蛋的蛋清与蛋黄的蛋白，通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离鸡蛋的蛋白质组份，采用免疫印迹(Western-blotting，WB)方法鉴定过敏原，通过离子交换层析对鸡蛋主要过敏原进行初步纯化。

The main components in soy protein isolate are 7S and 11S globulins. Studiesof papain on 7S, 11S and mixture of the two proteins suggested that 11S is themain component in SPI to form coagulation. 7S coagulation required higher papainaddition than 11S coagulation, while 11S gel's elastic modular was higher than7S gel. The elasticity of mixture depended on the proportion of 11S. Two proteinases were separated from papain by SP-sepharose cation-exchangechromatography.

首先分离出大豆蛋白中的主要组分7S和11S蛋白，将木瓜蛋白酶分别作用于两种蛋白的溶液和不同7S/11S比例的蛋白溶液，结果表明11S蛋白是大豆蛋白中形成酶促凝胶的主要组分；7S蛋白形成凝胶所用的酶浓度高于11S蛋白所用的酶浓度，但11S蛋白形成的凝胶的弹性强度却远高于7S蛋白；在两种蛋白的混合溶液中11S蛋白的比例对凝胶的弹性强度起决定作用。

The results showed that SFE-CO_2 was the ideal method to extract volatile oils from lotus leaves. GC-MS was applied to isolate and analyze the components of extracted volatile oils. 76 components were detected, among which SD and SE detected 23 components respectively, while SFE-CO_2 detected 52 components. The main ingredients were terpenes, alcohols, esters, ketones and alkyls, etc.

并通过GC-MS联用技术，对提取得到的挥发油组分进行了分离分析，共检出76种成分，其中水蒸气蒸馏法和有机溶剂提取法分别检出23种成分，超临界萃取所得荷叶挥发油检出52种成分，主要为萜烯、醇、酯和酮、烷类化合物等。

On the basis of the topological structure of FBB and their polymerization in borates(isolate, chain, layer and framework), a new systematic borate-classification is put forward,and all available borates are classified.

依据FBB自身的拓扑结构不同和在晶体结构中的排布方式不同(孤立、成链、成层、成三维网架)对所有可用的硼酸盐进行了系统的分类。

For all that, still thered are some steps with old and solid executive ideas of cabarets to isolate one-self today,only I only, considered as a business enterprise to fight of sacrifice article.

尽管如此，今天仍有一些酒店的经营者观念陈旧、固步自封、唯我独尊，把企业当做打斗的牺牲品。

Objective:To compare the response control and attention of Schizophrenic patients with that of the healthy controls by Integrated Visual and Auditorycontinuous performance test. To quantitate the impairment of cognitive function in patients, and to explore the relationship between cognitive function and the severity of the disorder.

目的：探讨精神分裂症病人在IVA持续操作测试中的反应控制能力、注意力等，并与健康人进行比较，从而量化精神分裂症病人的认知功能损害，并进一步探讨其认知功能与疾病严重程度的关系。

Main effective factors including subcooling degree, mechanical vibration, gas hydrate reformation, environment temperature, noncondensing gas and surfactant are analyzed.

指出过冷度、机械振动、重复生成水合物、环境温度、不凝性气体、添加剂是影响气体水合物生成的主要因素，还对R152a水合物的放冷进行了实验研究。