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Results After culture and reinfusion in vivo, CD 3 cells, CD 3 CD 8 cells, CD 3, CD 56 cells, cultured cells were significantly elevated, mDC and pDC significantly increased after transfusion, liver function had improved compared with before treatment .

结果 培养结束以及回输体内后， CD 3细胞、CD 3 CD 8细胞、CD 3 CD 8细胞较培养前显著升高，mDC 和pDC 在回输后也明显增高，肝脏功能较治疗前有所好转。

Restoration of ODC expression in the HL60-ODC cells resulted in rescue of rottlerin-induced apoptosis of HL60 cells. Simultaneously, phosphothreonine-ODC of rottlerin-treated HL60-ODC cells was highly elevated comparing with HL60-pcDNA3 cells. Taken together, we suggested that rottlerin, a PKCd inhibitor, might decrease phosphothreonine-ODC to reduce both protein stability and enzyme activity of ornithine decarbooxylase and finally resulted in cell apoptosis.

为了侦测鸟胺酸去羧化是否能够负调节rottlerin所引发的细胞凋亡，我们将具有鸟胺酸去羧化cDNA载体稳定转染至HL60细胞内，此细胞称为HL60-ODC细胞，另转染载体的细胞称为HL60-pcDNA3细胞；实验发现HL60-ODC细胞中的鸟胺酸去羧化蛋白为HL60-pcDNA3细胞与HL60细胞表现之2.5倍，同时HL60-ODC细胞提升鸟胺酸去羧化蛋白表现，能够减少rottlerin造成之细胞凋亡现象，并且此蛋白在HL60-ODC细胞中酥胺酸磷酸化较HL60-pcDNA3细胞表现高。

By culturing human brain microvascular endothelial cells and glioma cells separatedly and simutaneously ,and then analyzing their pre- and post-cultune FasL levels, we study the effect of the grouth of glioma cells upon FasL level of human brain microvascular endothelial cells.

我们通过将人脑毛细血管内皮细胞与胶质瘤细胞分别培养及共同培养，对培养前后人脑毛细血管内皮细胞与胶质瘤细胞FasL表达水平的分析，来探讨胶质瘤细胞的生长对人脑毛细血管内皮细胞FasL表达的影响。

Results: Follow-up ob servation of living cells revealed that upon the Ti disc there were cells migra ting from the cellular layer and attaching to the rim of it, forming cells bridg es between them, and then more cells were found to attach and orient as the cult ure went on.

结果：活细胞跟踪观察揭示钛片放入细胞层表面后即有细胞自细胞层表面向钛片侧壁移行、贴附，并在二者之间形成细胞桥。

Results: Follow-up ob servation of living cells revealed that upon the Ti disc there were cells migra ting from the cellular layer and attaching to the rim of it, forming cells bridg es between them, and then more cells were found to attach and orient as the cult ure wen

结果：活细胞跟踪观察揭示钛片放入细胞层表面后即有细胞自细胞层表面向钛片侧壁移行、贴附，并在二者之间形成细胞桥。

Under our conditions, detection rate of embryonic DNA were zero with 2 cells, 62.5% with 4 cells, 90% with 8 cells and 100% with 16 cells. A total of 23 embryos that had been biopsied and sexed were transferred into the donor...

对 2 3枚经过性别鉴定后的胚胎进行移植，获得 5头纯种试管牛，产犊率为 2 1 7%，与正常的胚胎移植结果 2 7 3%( 3 1 1 )无显著差异，预测性比为 1 0 0 %符合。

Methods At first, retrovirus vectors encoding IFN-γ or IL-4 gene were constructed. They were transfected into PA317 packaging cells by lipofectamine, PA317IFN-γ and PA317IL-4 cells were obtained. C6 glioma cells were infected with replication deficiency retrovius containing IFN-γ or IL-4 gene, cell morphology、cell growth curve and cloning efficiency assay were examined. The intracranial C6 glioma animal model was established in immunocompetent Wistar rats. PA317IFN-γ and PA317IL-4 cells were stereotactically implanted into the tumor areas alone or together. The survival of tumor bearing rats were examined, tumor volumes were measured and immunohistochemical analyses of CD4〓 and CD8〓 T lymphocyte infiltration were performed.

构建和鉴定携带IL-4或IFN-γ基因的逆转录病毒载体，将其导入逆转录病毒包装细胞PA317，通过G418抗性筛选，得到携带目的基因的包装细胞并鉴定；应用携带IL-4或IFN-γ基因的复制缺陷型逆转录病毒感染C6胶质瘤细胞，观察对C6胶质瘤细胞形态、细胞生长曲线和克隆形成率的影响；建立C6胶质瘤大鼠脑内移植动物模型，将携带IL-4和IFN-γ基因的逆转录病毒包装细胞分别或联合注射到脑内荷瘤大鼠的肿瘤组织中，观察其治疗作用，并初步探讨其机制。

To compare the growth characteristics of non-hematopoietic adult stem cells derived from rat fetal blood and rat bone marrow in vitro, and to study the differentiation of these stem cells into neuron-like cells in vitro,the fetal blood of pregnant rats and bone marrow of adult rats were sterilely collected; mononuclear cells were isolated by using standard Ficoll-hypague techniques and then cultured in DMEM/LG containing 10% fetal bovine serum.

为了比较大鼠胎血和骨髓中非造血成体干细胞（non-hematopoietic adult stem cells，NASC）体外培养过程中的生长特性及体外诱导两者向神经元样细胞分化的异同，在无菌条件下采集孕大鼠的胎血和成年大鼠的骨髓，用Ficoll-hypague分离液分离出单个核细胞后，种植于含10%胎牛血清的DMEM/LG培养液内。收获的NASC传代培养，用流式细胞仪检测细胞的免疫表型。

To compare the growth characteristics of non-hematopoietic adult stem cells derived from rat fetal blood and rat bone marrow in vitro, and to study the differentiation of these stem cells into neuron-like cells in vitro,the fetal blood of pregnant rats and bone marrow of adult rats were sterilely collected; mononuclear cells were isolated by using standard Ficoll-hypague techniques and then cultured in DMEM/LG containing 10% fetal bovine serum.

为了比较大鼠胎血和骨髓中非造血成体干细胞（non-hematopoietic adult stem cells，NASC）体外培养过程中的生长特性及体外诱导两者向神经元样细胞分化的异同，在无菌条件下采集孕大鼠的胎血和成年大鼠的骨髓，用Ficoll-hypague分离液分离出单个核细胞后，种植于含10%胎牛血清的DMEM/LG培养液内。

To compare the growth characteristics of non-hematopoietic adult stem cells derived from rat fetal blood and rat bone marrow in vitro, and to study the differentiation of these stem cells into neuron-like cells in vitro,the fetal blood of pregnant rats and bone marrow of adult rats were sterilely collected; mononuclear cells were isolated by using standard Ficoll-hypague techniques and then cultured in DMEM/LG containing 10% fetal bovine serum. The acquired NASCs were subcultured for passage.

为了比较大鼠胎血和骨髓中非造血成体干细胞（non-hematopoietic adult stem cells，NASC）体外培养过程中的生长特性及体外诱导两者向神经元样细胞分化的异同，在无菌条件下采集孕大鼠的胎血和成年大鼠的骨髓，用Ficoll-hypague分离液分离出单个核细胞后，种植于含10%胎牛血清的DMEM/LG培养液内。

Chinese traditional virtue is humility and wariness,the compliment and praise to the others should be declination

中国人的传统美德是谦虚谨慎，对别人的恭维和夸奖应是推辞。如

We bought this house on the never-never.

我们以分期付款的方式买下这座房子。