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Compared with the corresponding BEAS-2B cells, the shape of cell clone, the way of cell growth, the shape and quantity of cell and nuclear were not significantly changed in the 1st, 2nd, 5th passage BEAS-2BNNK cells. The shapes of cells and its nuclears of the 10th, 15th, 20th, 25th passage BEAS-2BNNK cells became round gradually, and the way of cell growth became from radio-form into membraniform and more than one nucleolus appeared.

BEAS-2BNNK细胞在第1、2、5代时细胞克隆形态及生长方式、细胞形态、细胞核形态及数目与相应代BEAS-2B无明显差异；第10、15、20、25代时细胞及胞核逐渐变圆，出现多个核仁，细胞由放射状生长逐渐变为膜状生长。

To mensurate the expression of MDR1 of different breast cancer cells from human breast cancer primary cells and MCF-7 breast cancer cell line,and the kill effect of chemotherapy drug with different breast cancer cells in vitro,and the change of the proporation and the expression of MDR1 of breast cancer stem cells after chemotherapy.

测定人乳腺癌标本和MCF-7中不同细胞亚群的MDR1表达情况；并测定化疗药物在体外对人乳腺癌原代细胞和MCF-7中不同细胞亚群的杀伤作用，以及在化疗药物干预后，乳腺癌干细胞的含量和MDR1表达的变化情况。

It shows that the embryogenic tissues are composed of a group of small meristematic cells at one end (embryo-proper cells), which are at different phages of cell division and many long cells at the other end (embryo-suspensor cells).

研究表明，胚性愈伤组织是由不同发育阶段的早期胚组成的细胞团，早期胚主要由长的胚柄细胞及其顶端的胚头细胞组成，而后者又具不同的分裂相。

Nestin-positive cells were mainly in the gastric mesenchyma and the neck region of the glands. In mucosa of small intestine, Musashi-1-positive cells and TERT-positive cells were mainly located between cell positions 4 and 7. Oct-4-positive cells were diffusely distributed in the crypts.

小肠黏膜中Musashi-1和TERT阳性细胞主要在距隐窝底部4~7个细胞的位置，Oct-4阳性细胞散在分布于腺体内，Nestin阳性细胞均分布在小肠黏膜间质，无腺体内着色。

Mesenchymal stem cells are multipotent cells derived from many adult tissues, which can differentiate into cells of the mesodermal lineage, such as adipocyte, osteocyte and chondrocyte, as well as cells of other embryonic lineages. They are a promising tool for tissue engineering.

间充质干细胞是一类可从多类成体组织中分离的能向脂肪细胞、成骨细胞和软骨细胞等分化的成体干细胞，是组织工程细胞的一个理想来源。

Human basic fibroblast growth factor is a single-chain heparin-binding polypeptide. It is a general regulatory mediator for different cells such as endothelial cells and for other cells of mesodermal and neuroectodermal origin, it stimulates cells proliferation, inducing or delaying differentiation.

人碱性成纤维细胞生长因子是一种单链的具有与肝素结合能力的多肽分子，它能够调节内皮细胞及其它来源于中胚层和神经外胚层等多种细胞的生长、分化、迁移和凋亡，同时是一种重要的血管生成因子，因此具有广泛的临床应用价值。

In mixoploid plants, somatic cells with 2n=25~29 were most frequent except for a few haploid cells (2n=19) and chromosome doubling cells (2n=50~60). Abnormal chromosome behaviors were observed in pollen mother cells from mixoploid BI-1 and haploid, including chromosome bridges, laggard chromosomes, meiotic cycle asynchrony and unequal separation of chromosomes.

对混倍体植株BI-1和单倍体植株的花粉母细胞(Pollen mother cells，PMCs)减数分裂染色体行为观察发现，高频率出现染色体落后、染色体桥、染色体分裂周期不同步、不均等分离等异常染色体行为。

Results: NKA-I cells was first found in the duodenum mucose on the 14th day of fetus, earlier than in the jejunum ( 16th day ) and ileum ( 17th day ), and its Positive reaction and the number of cells increased with further development, after birth the 30th day, it became quite identical to that of adult rat; NKA-I cells were seen in the colon mucosa on the 17th day of fetus, later on the number kept decreasing till grown up stage; No change was observed in the stomach mucosa except that few cells showed up on the 15th day of fetus; No positive cell was found on the oesphagus wall.

结果： NKA-I细胞首先见于胚胎14 d的十二指肠粘膜，早于空肠(胚胎16 d)和回肠(胚胎17 d)，其阳性反应强度及细胞量随发育而增加，30 d时与成年鼠相似；结肠粘膜内于胚胎17 d见细胞，但此后数量较少直至成年；胃粘膜于胚胎15 d见个别细胞外，以后无明显变化；食道壁始终未见阳性细胞。

Results: NKA-I cells was first found in the duodenum mucose on the 14th day of fetus, earlier than in the jejunum ( 16th day ) and ileum ( 17th day ), and its Positive reaction and the number of cells increased with further development, after birth the 30th day, it became quite identical to that of adult rat; NKA-I cells were seen in the colon mucosa on the 17th day of fetus, later on the number kept decreasing till grown up stage; No change was observed in the stomach mucosa except that few cells showed up on the 15th day of fetus; No positive cell was found on the oesphagus wall.

结果：NKA-I细胞首先见于胚胎14 d的十二指肠粘膜，早于空肠(胚胎16 d)和回肠(胚胎17 d)，其阳性反应强度及细胞数量随发育而增加，30 d时与成年鼠相似；结肠粘膜内于胚胎17 d见多个细胞，但此后数量较少直至成年；胃粘膜于胚胎15 d见个别细胞外，以后无明显变化；食道壁始终未见阳性细胞。

Methods: M-CSF-dependent bone marrow cells were isolated from 5-6 weeks old mice, and cultured in the presence of MCSF (25μg/L) with different concentrations of TNF-α(0, 1, 10, 100 μg/L) for 5 days, the formation of TRAP multinucleated cells was observed. These cells were also cultured in the presence of both RANKL (30 μg/L) and M-CSF (25 μg/L) with or without 10 μg/L TNF-α for4, 5, 6 and 9 days. The number of TRAP multinucleated cells and resorption pits on dentine slices were counted under light microscope.

选用小鼠巨噬细胞集落刺激因子依赖性非附着性骨髓细胞，在含有25μg/L M-CSF和0，1，10，100μg/L TNF-α的α-MEM培养液中培养5d后，观察抗酒石酸酸性磷酸酶染色阳性多核细胞的形成；细胞在含有25μg/L M-CSF和30μg/L sRANKL的α-MEM培养液中进行培养，比较加入和不加入10μg/L TNF-α培养4、5、6和9d后，所形成的TRAP多核细胞的数目和骨吸收面积。

Chinese traditional virtue is humility and wariness,the compliment and praise to the others should be declination

中国人的传统美德是谦虚谨慎，对别人的恭维和夸奖应是推辞。如

We bought this house on the never-never.

我们以分期付款的方式买下这座房子。