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METHODS Retinal cells were cultured in commixture and retinal ganglion cells in purification, to observe the state of ganglion cells and its axon. RESULTS Retinal ganglion cells that cultured in commixture grew very well.

方法进行猪视网膜细胞混合体外培养和神经节细胞的纯化培养，观察神经节细胞生长状态及轴突生长情况，研究视网膜不同类型神经细胞之间的相互作用。

Then we hypothesize that mesenchyma stem cells from DAC are heterology,which have the potential ability to regenerate root/periodontal complex structures as a new kind of seed cells. Therefore, this study focuses on characteristics of the mesenchyma stem cells at tooth root developing stage and the evaluation of the possibility of being seeds cells.

因此，本研究拟对牙根发育期相关间充质干细胞的生物学特性进行研究，探讨其作为种子细胞在牙根/牙周组织工程中应用的可行性，以期为组织工程化牙根/牙周组织构建优选新的种子细胞。

The cells labeled with PKH26 were seeded on the bio-derived bone to construct tissue engineered bone in vitro. Then the compound of cells and material were observed under fluorescence microscope. The compound of labeled cells and material were implanted into the rabbit thigh muscle, and the transformation of the labeled cells was observed by fluorescence microscope 14 and 28 days later.

将PKH26 标记的BMSCs 接种于生物衍生骨材料，48 h 后荧光显微镜观察细胞与材料的复合情况；将细胞-材料复合物植入成年新西兰大白兔双后肢肌袋，14、28 d 后荧光显微镜观察标记细胞的转归。

The suspension cells of the heart at E11 were respectively cultured in co-culture system based in the stromal cells from the heart, the fetal liver and AGM region with the same conceptus age, and c-kit+ cells were analyzed by flow cytometer, estimating the effect of different stromal cells on HSCs\' amplification.

将培养24h后的E11心脏悬浮细胞分别置入由同期的心脏内皮细胞、胎肝基质细胞和AGM区基质细胞构成的共培养体系中，于0h， 24h和48h分别检测c-kit+细胞比率，研究各组织基质细胞对HSCs扩增的影响。

The animal experiment stage: 40 rabbits underwent corneal epithelium curettement and limbal ablation to induce limbal stem cell deficiency and were randomly divided into four groups, simple amniotic membrane group, non-frozen cells group, glycerol group and dimethyl sulfoxide group(n=10 for each group). Searching for the activity of frozen and non-frozen cells more completely by transplanting subculturing cells and observing the accomplishment of cells function.

动物实验阶段：将40只角膜缘干细胞损伤的模型兔随机分成单纯羊膜组、未冷冻细胞组、DMSO保存细胞组和甘油保存细胞组，每组10只，用传代培养2周后的角膜缘上皮细胞作移植实验，观察细胞体外功能完成情况来进一步比较深低温保存前后角膜缘上皮细胞的活性。

Flow cytometry was used to measure the cells cycle kinetics of the treated cells. Hoechst33258/PI double staining was performed to evaluate the cell morphology. Western blotting was conducted to analyze the expressions of p53, Bcl 2 and Bax. Results 2 ClAdo and 2 CldAdo had different cytotoxicities on the cells, while 2' dAdo had no cytotoxicity on the cells.

以胎盼蓝活细胞拒染法测定药物对细胞生长、增殖的抑制及IC50 ；以流式细胞术观察药物对细胞周期的影响；以荧光双染法（Hoechst33258/PI）观察药物对细胞形态学的影响；以Western blot法观察药物对细胞P53、Bcl 2、Bax蛋白表达的影响。

At 8 weeks postoperatively, the grafts were mostly substituted by new cells, and the artificial ligament was completely parceled by the synovial membrane, but there were still some gaps; The combined tendon exhibited no loose or disfiguration, the infiltrative inflammatory cells disappeared. The cells in grafts were close to normal cells in anterior cruciate ligament, but fibrocytes arranged in disorder and fibers showed a verticality.

移植后第8周，移植物大部分组织被新长入的细胞替代，人工韧带被滑膜完全包裹，浸润的炎症细胞基本消失，部分接触面已融合，但部分面仍有间隙；联合肌腱无变形和松弛，移植物内细胞形态，数量接近正常前交叉韧带，但纤维细胞排列杂乱，纤维有了一定的纵向性。

Objective To explore the in vitro isolation of β2m(superscript -)/Thy-1(superscript +) bone marrow-derived liver stem cells which bear double features of stem and liver cells from bone marrow stem cells as so to provide suitable donor cells for the treatment of liver diseases by cellular transplant.

目的 尝试从骨髓干细胞中分选出具备干细胞和肝细胞双重特性的β2m/Thy-1骨髓源性肝干细胞亚群，为细胞移植治疗肝病提供合适的供体细胞。

The distribution of ABH substances in humantissue cells of nonsecretors differs from those insecretors.the contents of ABH in cells of nonsecretorswere much less than those of secretors.ABH substanceswere demonstred in cells of deep part of gastric gland,Brunner gland of duodenum,acini and duct of pancreas,acini and duct of sweat gland and in endothelial cells ofblood vessels and heart.

在国内外首次用免疫透射电镜对正常人体的胃、十二指肠、横结肠的粘膜上皮和腺上皮的ABH物质进行亚细胞定位研究后发现，ABH物质分布于粘膜上皮及粘液性细胞的Golgi器、粘原颗粒和胃腺主细胞的酶原颗粒中，证实人体组织细胞的ABH物质由自身产生，而不是从血液吸附。

The engraftment levels of cultured CD34+ cells were similar to those of fresh CD34+cells but the percentage of human lineage cells was higher than that of fresh CD34+ cells.

培养后CD34+细胞在小鼠体内的植入水平与新鲜CD34+细胞的相近，而其各系人源血细胞的含量高于新鲜CD34+细胞。

Objective:To compare the response control and attention of Schizophrenic patients with that of the healthy controls by Integrated Visual and Auditorycontinuous performance test. To quantitate the impairment of cognitive function in patients, and to explore the relationship between cognitive function and the severity of the disorder.

目的：探讨精神分裂症病人在IVA持续操作测试中的反应控制能力、注意力等，并与健康人进行比较，从而量化精神分裂症病人的认知功能损害，并进一步探讨其认知功能与疾病严重程度的关系。

Main effective factors including subcooling degree, mechanical vibration, gas hydrate reformation, environment temperature, noncondensing gas and surfactant are analyzed.

指出过冷度、机械振动、重复生成水合物、环境温度、不凝性气体、添加剂是影响气体水合物生成的主要因素，还对R152a水合物的放冷进行了实验研究。