查询词典 increasing sequence

The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2 -chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

Based on the analysis of the linear congruential generator and TigerSHARC20XS of ANALOGDEVICES,this paper presents a method for generating the pseudo random sequence in optional cycle by addressing the sequence of descending length.

文中基于线性同余算法，结合Analo Gdevices公司DSP芯片TigerSHARC20XS的运算结构，设计出一种利用寻址递减长度序列，从而产生具有遍历性的任意长度伪随机序列的方法。

Three uniform random sequences are built by the linear congruential method, of which two are used to form integer num, ber and decimal fraction parts of the new random sequence respectively and the third to shuffle the new sequence.

采用线性同余法生成3个均匀分布随机序列，其中两个分别组成新的随机序列的整数和小数部分，第3个用来对新序列进行混洗。

Strains YL001 and YL002 formed a monophyletic clade with strains of X. nematophilus with sequence homology outweighing 99% and the sequence homology to genus Photorhabdus outweighing 94%. The lag, logarithmic, stationary and contabescence phase of the two bacteria were 0-6, 6-18, 18-66 and 66 h respectively; pH of strains YL001 and YL002 reduced to 5.70 and 5.56 at 12 h, respectively, and then rose gradually to 7.74 and 8.07 respectively when culture finished. Glucose content reduced quickly during 0-18 h, and then kept stable. Amido nitrogen content reached the lowest at 12 h and then rose slowly. YL001 exhibited the highest inhibitory effects at 54 h and YL002 exhibited highest inhibitory effects at 42 and 66 h respectively on B. cirerea and B. subtilis. Strains YL001 and YL002 belonged to X.

YL001和YL002菌株与嗜线虫致病杆菌种内菌株形成一个类群，序列同源性大于99%，与发光杆菌属内菌株的序列同源性大于94%。2菌株的延缓期、对数生长期、稳定期和衰亡期分别为0~6，6~18，18~66和66 h；培养12 h后，YL001和YL002菌株发酵液的pH值分别降低至5.70和5.56，此后逐渐上升，至发酵结束时其pH值分别为7.74和8.07；培养0~18 h时2菌株发酵液中还原糖含量迅速降低，此后保持稳定；12 h时氨基氮含量达到最低，此后开始缓慢上升；YL001菌株培养54 h后及YL002菌株培养42和66 h后，其对番茄灰霉病菌和枯草芽孢杆菌的抑制作用最强。

Japonicus used in this experimentation is matemal inheritance.5 Sequence analysis of mitochondrial 16S rRNA and COI gene and molecular phylogeny of four species of sea cucumber500 bp 16S rRNA and 540 bp COI fragment were obtained by PCR method from mitochondrial DNA of four species of sea cucumber, Apostichopus japonicus, Holothuria fuscogilva, Cucumaria frondosa and Mensamaria intercedens. Variable sites, nucleotide diversity, average number of nucleotide differences and average number of nucleotide substitutions per site were calculated to analyze sequence difference, and phylogenetic trees were constructed by the NJ and ME method.

结果显示在来自这5组家系的100个后代中仅检测到母本的单倍型，刺参的线粒体遗传模式表现为典型的母系遗传。5 4种海参16S rRNA和COI基因片段序列比较及系统学研究采用PCR技术对山东荣成、长岛、俄罗斯和日本的刺参，黄乳海参，北大西洋瓜参，二色桌片参的16S rRNA和COI基因片段进行了扩增和测序，分别得到了长度约为500 bp和540 bp的片段。

After the multiple sequence alignments, the sequence variation analyses were conducted and the molecular phylogenetic trees of Cypraeidae were reconstructed with NJ, MP and bayesian methods, using Prosimnia semperi of Cypraeacea as an outgroup.

其次，用自测的宝贝科11种软体动物的mtDNA COI基因序列，结合得自GenBank上的18种宝贝科软体动物的同源序列，用NJ法、ML法以及贝叶斯法构建了分子系统树。

Full-length cDNA sequence of NPY from Barbodes sinensis Based on multiple alignment of a number of Cyprinidae NPY genes, two special primers of 3'cDNA ends were designed for amplification of the 3'cDNA ends of NPY genes and the sequence was acquired from the cDNA templet of the brain of S.sinensis.

中华倒刺鲃基因推导氨基酸序列的分析中华倒刺鲃NPY的cDNA编码区核苷酸长度为291bp，编码96个氨基酸，由信号肽(28个氨基酸)、NPY成熟肽(36个氨基酸)、三肽加工位点(3个氨基酸)和连接肽(29个氨基酸)组成。

The results of 16S rRNA sequence and phylogenetic analysis show that the strain is the closest relative to Desulfovibrio sp. with 96.0% sequence similarity.

基于16SrDNA序列同源性构建了系统发育树，结果表明此株菌是属于脱硫弧菌属的硫酸盐还原菌，与Desulfovibrio具有96.0%的序列相似性。

The sequence of Diurnal change of WUEph of different plants in lawn community is: Geranium sibiricum, Leymus secalinus, Potentilla supine., Viola prionantha, Poa pratensis and Potentilla anserine:The sequence of physiological drought resistance of weeds and turf in lawn community is: Poa pratensis, Digitaria ischaemumjotentilla supine, Potentilla anserine, Viola prionantha, Geranium sibiricum and Inula britanica.

草坪群落中不同植物的水分利用效率日变化均值大小为：鼠掌老鹳草＞赖草＞铺地委陵菜＞早开堇菜＞草地早熟禾＞鹅绒委陵菜。

Methods:64 cases of intraspinal spacc-occupying discase were examined by conventional myelography,CT myelography and MRI,CTM were performed after 6 hours of myelography,MRI were performed with SE sequence and combined with GE sequence.

脊髓造影采用常规脊髓造影和 CTM相结合。CTM在常规脊髓造影后 4～ 6 h进行。 MRI采用 SE序列加 GE序列。椎管内占位性病变的病灶检出率、定位和定性准确率做一统计和分析。

"Second Life is remarkably easy to work with, and is very popular,"

"第二次生命是显着容易的工作，并且很受欢迎，"

For example, we usually assume that materials are homogeneous and isotropic and free of internal defects or flaws.

为了得到适合有限元分析的模型，我们必须经过如图2所示的简化步骤。