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In neuroepithelial tumors, Cx43\, Cx32 expression varies greatly. Cx43 expression is related to histopathological type and degree of malignant of tumors.

神经上皮组织起源的脑肿瘤中CX表达可下降或缺失，Cx43表达与肿瘤的病理类型及恶性程度密切相关。

METHODS:Nestin expression was detected in 33 cases of neuroepithelial tumors using the immunocytochemical staining. The Nestin positive and negative cells were isolated by magnetic activated cell sorting. Nestin positive cells were culutered, identified, and induced to differentiate. The expression of Nestin protein was detected in Nestin+ cells by RTPCR.

采用免疫组化法检测神经上皮肿瘤组织中Nestin的表达情况，免疫磁珠法分选肿瘤组织中的Nestin+细胞，悬浮细胞培养法进行Nestin+细胞的增殖、分化与鉴定，RTPCR法检测Nestin蛋白在其中的活性表达情况。

Objective To study c-fos expression and apoptosis after traumatic brain injury in rats, and to explore the role of apoptosis in the secondary brain injury ,and the relation between c-fos expression and neuroglial apoptosis.

目的研究创伤性脑损伤后脑组织c-fos表达及神经细胞凋亡，探讨神经细胞凋亡在脑继发性损害中的作用，及c-fos基因表达与凋亡的关系。

Objective To study cfos expression and apoptosis after traumatic brain injury in rats,and to explore the role of apoptosis in the secondary brain injury,and the relation between cfos expression and neuroglial apoptosis.

目的 研究创伤性脑损伤后脑组织cfos表达及神经细胞凋亡，探讨神经细胞凋亡在脑继发性损害中的作用，及cfos基因表达与凋亡的关系。

Objective To study c fos expression and apoptosis after traumatic brain injury in rats,and to explore the role of apoptosis in the secondary brain injury,and the relation between c fos expression and neuroglial apoptosis.

目的 研究创伤性脑损伤后脑组织c fos表达及神经细胞凋亡，探讨神经细胞凋亡在脑继发性损害中的作用，及c fos基因表达与凋亡的关系。

objective To study cfos expression and apoptosis after traumatic brain injury in ratsand to explore the role of apoptosis in the secondary brain injuryand the relation between cfos expression and neuroglial apoptosis.

目的 研究创伤性脑损伤后脑组织cfos表达及神经细胞凋亡，探讨神经细胞凋亡在脑继发性损害中的作用，及cfos基因表达与凋亡的关系。

The experimental study is to explore the effect about rhEPO on neuromotor function, secondary pathology change and expression of NTF and the apoptosis of neural cell, which administered at different time after traumatic spinal cord injury. The experimental study is devided into three parts: The first part The effect of rhEPO on NT-3 and NGF expression after traumatic spinalcord injury Objective Nerve growth factor and neurotrophic factor–3(NT-3) are chief members in neurotrophic factors family, which can promote nerve cell survival、growth and differentiation.

本研究拟在脊髓损伤后不同时间应用促红细胞生成素干预，探讨其对神经运动功能和继发性病理改变的保护作用及对神经营养因子表达和神经细胞凋亡的影响，共分为以下三个部分：第一部分脊髓损伤后促红细胞生成素对NGF、NT-3表达的影响目的：神经生长因子和神经营养因子-3(NT-3)是神经营养因子家族的主要成员，是机体产生的能够促进神经细胞存活、生长、分化的一类多肽或蛋白质因子。

In contrast, exposure to ectopically expressed noncanonical Wnt11 markedly decreased the expression of Oct-4 and Nanog and induced mRNA expression (quantitative real-time reverse-transcription polymerase chain reaction) of cardiac-specific genes (Nkx2.5, GATA-4, atrial natriuretic peptide,- and -myosin heavy chain, and cardiac troponin T) by day 3 with subsequent progression to a pattern characteristic of the cardiac fetal gene program.

对比之下，接触到非典型wnt11能明显降低Oct-4 和 Nanog表达和在第3天出现诱导心脏特异性基因（Nkx2.5，GATA - 4，心房利钠肽，- and -肌球蛋白重链，肌钙蛋白T）mRNA的表达（应用反转录聚合酶链式反应实时定量），随后逐渐表达为心脏胎儿基因。

Light microscope and transmission electron microscopy showed that SMMC-7721 cells induced by SAHA had undergone the restorational alteration in morphology and ultrastructure, which were different from those of nontreated cells but were similar to those of normal cells, and the changes were as follows: the cells turned to be flat and spread; the nucleo-cytoplasmic ratio lessened and nuclear shape became rather regular; the number of nucleolus reduced and its volume lessened; euchromatin increased while heterochromatin decreased in nucleus; in the cytoplasm, mitochondria grew in number with relatively consistent structure and well-developed mitochondria cristae; Golgi complex turned to be well-developed and typical; rough endoplasmic reticulum increased. Immunocytochemistry assay showed that the expression of AFP and PCNA were declined significantly. FCM analysis showed SAHA could arrest SMMC-7721 cells in G0/G1 phase, with an accumulation of the cells in G0/G1 phase while a decrease of cells in S phase. Semi-quantitative RT-PCR detection revealed that the expression of p21WAFl mRNA was upregulated remarkably in the cells treated with SAHA.

结果：倒置显微镜和透射电镜观察显示，经SAHA处理的细胞增殖速度显著减慢，细胞体积增大，细胞核较小，形状较为规则，核仁数量减少、体积变小，核内常染色质增多而异染色质减少，核质比例减小，细胞质内线粒体数量增多、线粒体嵴发达，高尔基体较为典型，粗糙型内质网增多，呈现出与正常上皮细胞相似的形态变化；MTT比色法测定结果显示不同浓度(2.5、5.0、7.5、10.0uM)SAHA对SMMC-7721细胞的增殖均有抑制作用，并有明显的剂量依赖和时间依赖关系；免疫细胞化学检测显示SAHA能显著降低PCNA和AFP在SMMC-7721细胞中的表达；流式细胞仪检测结果显示，SMMC-7721细胞经SAHA处理后，G0/G1期细胞明显增加，S期细胞则明显减少，细胞被阻滞于G0/G1期；RT-PCR检测结果表明，SAHA作用12h后SMMC-7721细胞中p21WAF1 mRNA的表达即有增加，24h后更为明显。

There was a stable and relatively low expression level for Lbα1 and a progressive expression pattern for Lbα8 among the 1st,2nd,3rd,and 4th nymphal stages.5 Development and reproduction of L.decolor as a function of temperatureThe developmental rate,survival,intrinsic rate of increase,net reproductive rate(R_0),mean generation time,and population doubling time of L.decolor population were evaluated at eight constant temperatures.

Lb a1在1龄、2龄、3龄、4龄若虫及成虫中的表达水平分别是Lb a8的2.03、2.55、4.77、6.54、和5.28倍，且它们之间的差异均达到显著水平(P＜0.05)。5温度对无色书虱发育和繁殖的影响通过比较无色书虱在不同温度条件下的发育速率、存活率、内禀增长率等生命参数以明确无色书虱在不同温度条件下的发生发展规律。

"Second Life is remarkably easy to work with, and is very popular,"

"第二次生命是显着容易的工作，并且很受欢迎，"

For example, we usually assume that materials are homogeneous and isotropic and free of internal defects or flaws.

为了得到适合有限元分析的模型，我们必须经过如图2所示的简化步骤。