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According to the eresults described above, we preliminarily suggested that the pathogenic mechanism of Clonorchis sinensis may be related to activation of PKC and increased expression of γ-GTase.Praziquantel, not only can destroy adult worm directly, but also regulate γ-GTase activity. Furthermore, by inhibiting the expression of PKC, Praziquantel can elevate the repair of cells and reduce tumorigenesis. Further investigations are needed to dissect the detailed mechanism.

根据以上的实验结果，我们初步推测中华肝吸虫的致病机转可能和 PKC 的活化作用、γ-GTase 活性的表现有关，而驱虫药 Praziquantel 治疗中华肝吸虫，除了直接破坏虫体外，也可能会抑制 PKC 的表现进而调节γ-GTase 的活性，藉以提升细胞的免疫修复，而降低癌化的产生；唯其详细机转有待进一步探讨。

Results: Through analysis gene expression profiling of cholangiocarcinoma metastasis tissues and normal tissues, 232 genes differentially expressed in 4 samples were identified, 8 genes expression up-regulated and 6 genes down-regulated coinstantaneous.

结果 胆管转移癌与正常胆管粘膜的基因表达谱分析，有232条基因表达有明显差异，与转移相关基因一致的表达14条，8条表达上调，6条表达下调。

Methods:The total RNA was extracted from mouse RAW264.7 cells stimulated by LPS.The sequence including the whole length of HMGB1 was amplified by RT-PCR and inserted into pMD-19T.The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2+-chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2 -chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

UPA and PAT-1 might be the target genes of Smad4. It was much more significant to investigate the expression of Smad4 uPA and PAI-1 combinedly in colorectal carcinoma than to investigate the expression of only one of them.

联合检测大肠癌中 Smad4、uPA和PAI-1蛋白的表达情况，相比单一检测一种蛋白而言，对于评估大肠癌的发生、发展及预后更具有意义。

Based on these previous work, in this research, in order to explore the the molecular mechanism of enhanced tumor cell immunogenicity treated by elemene and mytomycin C compositely, we used murine Hca-F ascitic hepatic carcinoma ( high lymphatic metastasis substrain of H22) as an experimental model to compare the immunoprotective effects on Hca-F cells as well as the mechanism of the effects caused by HSP70-peptide complex purified from untreated tumor cells, EC-TCV and BCG; We studied the mechanism of increased tumor cell membrane HSP expression treated with elemene by the techniques of RT-PCR and immunoelectron microscopy; In order to deeply elucidate the antitumor effects and the molecular mechanism of elemene in increasing tumor cell immunogenicity we analyzed and compared the effects of elemene and heat shock on the gene expressions of tumor cell using gene expression profile chip.

本论文在上述研究成果的基础上，选择小鼠肝癌H22的高淋巴道转移亚系Hca-F为模型，进一步比较了未处理肿瘤细胞来源、榄香烯复合瘤苗来源的和卡介苗来源的HSP70-肽复合物对Hca-F的免疫保护效应、效应机制，探讨榄香烯-丝裂霉素复合处理增强肿瘤细胞免疫原性的分子机制；以Hca-F和人肝癌细胞株HepG〓等为模型研究了榄香烯处理增强肿瘤细胞膜热休克蛋白表达的机制；以HepG〓为模型用表达谱基因芯片研究比较了榄香烯与热休克处理对肿瘤细胞基因表达的影响，从而进一步阐明榄香烯抗肿瘤作用及增强肿瘤细胞免疫原性的分子机制。

Defective expression of CR1 in liver cancer is mostly acquired through central peripheral mechanisms.The changes of CR1 quantitative expression and adhering activity are consanguineously related to the severity and the activity of the development in patients with liver cancer.

结论提示肝癌患者红细胞CR1分子数量及粘附活性的变化主要是后天因素引起，肝癌患者红细胞CR1分子数量及粘附活性的变化与病情发展及严重程度密切相关。

However, the expression of Ku gene is constitutively at high levels in mammalian cells, although several studies suggest that expression of Ku is dependent upon an entry into the cell cycle.

在最近的研究中，已发现在高等植物阿拉伯芥中也发现Ku70和Ku80的同源基因(AtKu70和AtKu80)，并且它们与哺乳动物中的Ku具有类似的功能。

That is to say, when we are weighing the contemporariness of a work, only the seeming "expression" is never enough, we have to further examine the effectiveness of its "expression"--is the question new, is the perspective unique and the reflection profound.

也就是说，我们在衡量艺术品的当代性时，不是看似有&表达&就可以了，还要进一步追问作品&表达&的有效性--其问题是否新颖、视角是否独特、思考是否深刻。

GdCl3(10mg/kg) or the same volume of NS was continually injected of vein at 48h and 24h before LPS(5mg/kg) was injected in the male kunming species mouse. Then took out of liver or isolated KCs 30 minute after LPS was injected and assay the protein expression and phosphorylation level of ERK1/2 and p38MAPK of liver or KCs in vivo.Ⅱ. Isolated and cultured KCs of mouse, 1 h pretreatment with GdCl3; Culture medium with LPS(100ng/ml) was added and continuatively incubated 30 minute. Assay the protein expression and phosphorylation level of ERK1/2 and p38MAPK of KCs in vitro and detection effect of GdCl3 on its phagocytosis function in respectively.

Ⅰ 雄性昆明种小鼠在注射LPS（5mg/kg）前48h、24h，分别静脉注射GdCl3（10mg/kg ）或等量的生理盐水，于LPS或生理盐水注射后30min，分别取出肝脏或分离KCs，检测肝脏或KCs ERK1/2、p38MAPK蛋白表达及磷酸化水平；Ⅱ体外培养小鼠KCs经GdCl3（100μM）预处理1h，加入含LPS（100ng/ml）的DMEM培养基继续孵育30min，分别检测体外培养KCs ERK1/2、p38MAPK蛋白表达及磷酸化水平和GdCl3对其吞噬、分泌功能的影响。

"Second Life is remarkably easy to work with, and is very popular,"

"第二次生命是显着容易的工作，并且很受欢迎，"

For example, we usually assume that materials are homogeneous and isotropic and free of internal defects or flaws.

为了得到适合有限元分析的模型，我们必须经过如图2所示的简化步骤。